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Projekt Druckansicht

Aufklärung der Membraninsertionsmechanismen selbstinsertierender Membranproteine mithilfe von Ensemble- und Einzelmolekülspektroskopie

Fachliche Zuordnung Biophysik
Biochemie
Förderung Förderung von 2015 bis 2019
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 276454827
 
Erstellungsjahr 2021

Zusammenfassung der Projektergebnisse

Within this project, we have established various methodological advances from time-resolved spectroscopy to single-molecule FRET for membrane protein folding. Exploiting these methods, we have learned many fundamental insights on membrane-protein structure and folding, in particular for the membrane-associated protein Mistic and OmpLA. Morevoer, we exploited our developments also beyond these two model proteins and applied our techniques to obtain fundamental insights into transmembrane a-helix hairpin structures in lipid bilayers, also with regard to disease mutations. Besides our groups, also the field advanced within the past years to study structural dynamics of membrane proteins. This project was key to developing various methodologies, which the Schlierf lab is currently using to study the interaction of outer membrane proteins with periplasmic chaperones and a project funded by the Mukoviszidose e.V. on correction of CFTR transmembrane segment folding with small molecules.

Projektbezogene Publikationen (Auswahl)

 
 

Zusatzinformationen

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