Rolle des Sgst1/Top3/Rmi1-Komplexes bei der Reparatur steckengebliebener Replikationsgabeln in der Hefe Saccharomyces Cerevisiae

• Antragstellerin: Dr. Anna Heidenblut
• Fachliche Zuordnung: Allgemeine Genetik und funktionelle Genomforschung
• Förderung: Förderung von 2006 bis 2007
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 26661009

Genome instability is a hallmark of cancer cells. In healthy cells the stability of the genome is maintained by several classes of proteins, one of which is the family of so-called RecQ helicases. RecQ helicases unwind double-stranded DNA and one of the functions of these proteins is to restart DNA replication when replication has stalled due to DNA damage. The budding yeast RecQ helicase, Sgs1, forms a complex with Top3, a protein that can alter the topology of DNA, and Rmi1, a DNA binding protein. Yeast strains lacking Top3 or Rmi1 grow much more slowly than normal yeast strains, which is probably due to a toxic DNA replication intermediate that accumulates in the absence of either of these proteins. One aim of this research project is to elucidate the nature of this toxic DNA structure and whether it also accumulates in yeast strains lacking proteins other than Top3 or Rmi1 that operate in the same replication fork recovery pathway. Another aim of this project is to identify proteins that interact with Rmi1.

• DFG-Verfahren: Forschungsstipendien
• Internationaler Bezug: Großbritannien
• Gastgeber: Professor Dr. Ian D. Hickson