Project Details
Projekt
Rolle des Sgst1/Top3/Rmi1-Komplexes bei der Reparatur steckengebliebener Replikationsgabeln in der Hefe Saccharomyces Cerevisiae
Applicant
Dr. Anna Heidenblut
Subject Area
General Genetics and Functional Genome Biology
Term
from 2006 to 2007
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 26661009
Genome instability is a hallmark of cancer cells. In healthy cells the stability of the genome is maintained by several classes of proteins, one of which is the family of so-called RecQ helicases. RecQ helicases unwind double-stranded DNA and one of the functions of these proteins is to restart DNA replication when replication has stalled due to DNA damage. The budding yeast RecQ helicase, Sgs1, forms a complex with Top3, a protein that can alter the topology of DNA, and Rmi1, a DNA binding protein. Yeast strains lacking Top3 or Rmi1 grow much more slowly than normal yeast strains, which is probably due to a toxic DNA replication intermediate that accumulates in the absence of either of these proteins. One aim of this research project is to elucidate the nature of this toxic DNA structure and whether it also accumulates in yeast strains lacking proteins other than Top3 or Rmi1 that operate in the same replication fork recovery pathway. Another aim of this project is to identify proteins that interact with Rmi1.
DFG Programme
Research Fellowships
International Connection
United Kingdom
