FOR 1082:  Biochemistry and Biological Function of Dnmt2 Methyltransferases

• Subject Area: Biology
• Term: from 2009 to 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 65636347

• Final Report Year: 2016

Final Report Abstract

No abstract available

Publications

• Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. Nature Genetics, Vol. 41. 2009, pp. 696–702.
  Phalke, S., Nickel O., Walluscheck D., Hortig F., Onorati M.C., Reuter G.
  (See online at https://doi.org/10.1038/ng.360)
• In vitro tRNA methylation assay with Entamoeba histolytica DNA and tRNA methyltransferase Dnmt2 (Ehmeth) enzyme. Journal of Visualized Experiments, Vol. 44. 2010: 2390.
  Tovy, A., Hofmann, B., Helm, M., Ankri, S.
  (See online at https://dx.doi.org/10.3791/2390)
• RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage. Genes & Development, Vol. 24.2010, pp. 1590-1595.
  Schaefer M., Pollex T., Hanna K., Tuorto F., Meusburger M., Helm M., Lyko F.
  (See online at https://dx.doi.org/10.1101/gad.586710)
• Glucose starvation boosts Entamoeba histolytica virulence. PLOS Neglected Tropical Diseases, Vol. 5. 2011, Issue 8, e1247.
  Tovy A., Hertz R., Siman-Tov R., Syan S., Faust D., Guillen N., Ankri S.
  (See online at https://doi.org/10.1371/journal.pntd.0001247)
• Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L. Journal of Biological Chemistry, Vol. 286. 2011, pp. 24200-24207.
  Jurkowska, R.Z., Rajavelu, A., Anspach, N., Urbanke, C., Jankevicius, G., Ragozin, S., Nellen, W., Jeltsch, A.
  (See online at https://dx.doi.org/10.1074/jbc.M111.254987)
• Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Research, Vol. 40. 2012, Issue 22, pp. 11648–11658.
  Becker M., Müller S., Nellen W., Jurkowski T.P., Jeltsch A., Ehrenhofer-Murray A.E.
  (See online at https://doi.org/10.1093/nar/gks956)
• RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis. Nature Structural & Molecular Biology, Vol. 19. 2012, pp. 900–905.
  Tuorto F., Liebers R., Musch T., Schaefer M., Hofmann S., Kellner S., Frye M., Helm M., Stoecklin G., Lyko F.
  (See online at https://doi.org/10.1038/nsmb.2357)
• Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA). Nucleic Acids Research, Vol. 41. 2013, Issue 18, pp. 8615–8627.
  Müller S., Windhof I.M., Maximov V., Jurkowski T., Jeltsch A., Förstner K.U., Sharma C.M., Gräf R., Nellen W.
  (See online at https://doi.org/10.1093/nar/gkt634)
• The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in Drosophila. Cell Reports, Vol. 4. 2013, Issue 5, pp. 931-937.
  Durdevic Z., Mobin M.B., Hanna K., Lyko F., Schaefer M.
  (See online at https://doi.org/10.1016/j.celrep.2013.07.046)
• Stress granule formation in Entamoeba histolytica: cross-talk between EhMLBP, EhRLE3 reverse transcriptase and polyubiquitinated proteins. Cellular Microbiology, Vol. 16. 2014, Issue 8, pp. 1211-1223.
  Katz S., Trebicz-Geffen M., Ankri S.
  (See online at https://doi.org/10.1111/cmi.12273)
• The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Research, Vol. 42. 2014, Issue 10, pp. 6487–6496.
  Shanmugam R., Aklujkar M., Schäfer M., Reinhardt R., Nickel O., Reuter G., Lovley D.R., Ehrenhofer-Murray A., Nellen W., Ankri S., Helm M., Jurkowski T.P., Jeltsch A.
  (See online at https://doi.org/10.1093/nar/gku256)
• Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine. Nucleic Acids Research, Vol. 43. 2015, Issue 22, pp. 10952–10962.
  Müller M., Hartmann M., Schuster I., Bender S., Thüring K.L., Helm M., Katze J.R., Nellen W., Lyko F., Ehrenhofer-Murray A.E.
  (See online at https://doi.org/10.1093/nar/gkv980)
• RNA 5-Methylcytosine Analysis by Bisulfite Sequencing. Methods in Enzymology, Vol. 560. 2015, pp. 297-329.
  Schaefer M.
  (See online at https://doi.org/10.1016/bs.mie.2015.03.007)
• Proteomic Identification of Oxidized Proteins in Entamoeba histolytica by Resin-Assisted Capture: Insights into the Role of Arginase in Resistance to Oxidative Stress. PLOS Neglected Tropical Diseases, Vol. 10. 2016, Issue 1: e0004340.
  Shahi P., Trebicz-Geffen M., Nagaraja S., Alterzon-Baumel S., Hertz R., Methling K., Lalk M., Ankri S.
  (See online at https://doi.org/10.1371/journal.pntd.0004340)