FOR 1082:  Biochemistry and biological function of Dnmt2 methyltransferases

• Fachliche Zuordnung: Biologie
• Förderung: Förderung von 2009 bis 2016
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 65636347

• Erstellungsjahr: 2016

Zusammenfassung der Projektergebnisse

Keine Zusammenfassung vorhanden

Projektbezogene Publikationen (Auswahl)

• Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. Nature Genetics, Vol. 41. 2009, pp. 696–702.
  Phalke, S., Nickel O., Walluscheck D., Hortig F., Onorati M.C., Reuter G.
  (Siehe online unter https://doi.org/10.1038/ng.360)
• In vitro tRNA methylation assay with Entamoeba histolytica DNA and tRNA methyltransferase Dnmt2 (Ehmeth) enzyme. Journal of Visualized Experiments, Vol. 44. 2010: 2390.
  Tovy, A., Hofmann, B., Helm, M., Ankri, S.
  (Siehe online unter https://dx.doi.org/10.3791/2390)
• RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage. Genes & Development, Vol. 24.2010, pp. 1590-1595.
  Schaefer M., Pollex T., Hanna K., Tuorto F., Meusburger M., Helm M., Lyko F.
  (Siehe online unter https://dx.doi.org/10.1101/gad.586710)
• Glucose starvation boosts Entamoeba histolytica virulence. PLOS Neglected Tropical Diseases, Vol. 5. 2011, Issue 8, e1247.
  Tovy A., Hertz R., Siman-Tov R., Syan S., Faust D., Guillen N., Ankri S.
  (Siehe online unter https://doi.org/10.1371/journal.pntd.0001247)
• Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L. Journal of Biological Chemistry, Vol. 286. 2011, pp. 24200-24207.
  Jurkowska, R.Z., Rajavelu, A., Anspach, N., Urbanke, C., Jankevicius, G., Ragozin, S., Nellen, W., Jeltsch, A.
  (Siehe online unter https://dx.doi.org/10.1074/jbc.M111.254987)
• Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Research, Vol. 40. 2012, Issue 22, pp. 11648–11658.
  Becker M., Müller S., Nellen W., Jurkowski T.P., Jeltsch A., Ehrenhofer-Murray A.E.
  (Siehe online unter https://doi.org/10.1093/nar/gks956)
• RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis. Nature Structural & Molecular Biology, Vol. 19. 2012, pp. 900–905.
  Tuorto F., Liebers R., Musch T., Schaefer M., Hofmann S., Kellner S., Frye M., Helm M., Stoecklin G., Lyko F.
  (Siehe online unter https://doi.org/10.1038/nsmb.2357)
• Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA). Nucleic Acids Research, Vol. 41. 2013, Issue 18, pp. 8615–8627.
  Müller S., Windhof I.M., Maximov V., Jurkowski T., Jeltsch A., Förstner K.U., Sharma C.M., Gräf R., Nellen W.
  (Siehe online unter https://doi.org/10.1093/nar/gkt634)
• The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in Drosophila. Cell Reports, Vol. 4. 2013, Issue 5, pp. 931-937.
  Durdevic Z., Mobin M.B., Hanna K., Lyko F., Schaefer M.
  (Siehe online unter https://doi.org/10.1016/j.celrep.2013.07.046)
• Stress granule formation in Entamoeba histolytica: cross-talk between EhMLBP, EhRLE3 reverse transcriptase and polyubiquitinated proteins. Cellular Microbiology, Vol. 16. 2014, Issue 8, pp. 1211-1223.
  Katz S., Trebicz-Geffen M., Ankri S.
  (Siehe online unter https://doi.org/10.1111/cmi.12273)
• The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Research, Vol. 42. 2014, Issue 10, pp. 6487–6496.
  Shanmugam R., Aklujkar M., Schäfer M., Reinhardt R., Nickel O., Reuter G., Lovley D.R., Ehrenhofer-Murray A., Nellen W., Ankri S., Helm M., Jurkowski T.P., Jeltsch A.
  (Siehe online unter https://doi.org/10.1093/nar/gku256)
• Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine. Nucleic Acids Research, Vol. 43. 2015, Issue 22, pp. 10952–10962.
  Müller M., Hartmann M., Schuster I., Bender S., Thüring K.L., Helm M., Katze J.R., Nellen W., Lyko F., Ehrenhofer-Murray A.E.
  (Siehe online unter https://doi.org/10.1093/nar/gkv980)
• RNA 5-Methylcytosine Analysis by Bisulfite Sequencing. Methods in Enzymology, Vol. 560. 2015, pp. 297-329.
  Schaefer M.
  (Siehe online unter https://doi.org/10.1016/bs.mie.2015.03.007)
• Proteomic Identification of Oxidized Proteins in Entamoeba histolytica by Resin-Assisted Capture: Insights into the Role of Arginase in Resistance to Oxidative Stress. PLOS Neglected Tropical Diseases, Vol. 10. 2016, Issue 1: e0004340.
  Shahi P., Trebicz-Geffen M., Nagaraja S., Alterzon-Baumel S., Hertz R., Methling K., Lalk M., Ankri S.
  (Siehe online unter https://doi.org/10.1371/journal.pntd.0004340)