Identification of novel Fanconi Anemia pathway-regulated DNA repair factors

• Antragsteller: Dr. Lucian Moldovan
• Fachliche Zuordnung: Zellbiologie
• Förderung: Förderung von 2007 bis 2008
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 63157533

The Fanconi Anemia DNA repair pathway coordinates the removal of DNA crosslinks during replication, its inactivation causing a severe cancer susceptibility syndrome. An essential step in removal of crosslinks is monoubiquitination of the FA proteins D2 and I, leading to the formation of repair foci, however the mechanism employed is unknown. I plan to investigate the molecular basis of FAdependent DNA repair by focusing on the identification of new members of the pathway based on their ability to interact with activated D2 and I, and their subsequent characterization. Moreover, using biochemical, genetic and microscopy studies, I will examine the cooperation between FA and other repair mechanisms, in order to reveal why inactivation of the Fanconi pathway leads to decreased mutagenesis. These experiments are expected to reveal important insights into the mechanisms by which FA activation controls the repair of DNA crosslinks, and how deregulation of this essential genome maintenance mechanism leads to cellular transformation.

• DFG-Verfahren: Forschungsstipendien
• Internationaler Bezug: USA
• Gastgeber: Professor Dr. Alan D. D' Andrea