Project Details
Projekt
Identification of novel Fanconi Anemia pathway-regulated DNA repair factors
Applicant
Dr. Lucian Moldovan
Subject Area
Cell Biology
Term
from 2007 to 2008
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 63157533
The Fanconi Anemia DNA repair pathway coordinates the removal of DNA crosslinks during replication, its inactivation causing a severe cancer susceptibility syndrome. An essential step in removal of crosslinks is monoubiquitination of the FA proteins D2 and I, leading to the formation of repair foci, however the mechanism employed is unknown. I plan to investigate the molecular basis of FAdependent DNA repair by focusing on the identification of new members of the pathway based on their ability to interact with activated D2 and I, and their subsequent characterization. Moreover, using biochemical, genetic and microscopy studies, I will examine the cooperation between FA and other repair mechanisms, in order to reveal why inactivation of the Fanconi pathway leads to decreased mutagenesis. These experiments are expected to reveal important insights into the mechanisms by which FA activation controls the repair of DNA crosslinks, and how deregulation of this essential genome maintenance mechanism leads to cellular transformation.
DFG Programme
Research Fellowships
International Connection
USA
