Charakterisierung und Kartierung von "quantitative trait loci" (QTL) bei ostafrikanischen Buntbarschen der Tribus Haplochromini
Zusammenfassung der Projektergebnisse
Cichlid fishes are an evolutionarily very successful group (Family) of fishes that have given rise to an astonishingly large number of species and formed species flocks in record breaking time. We set out to study the genome of these cichlids by constructing a (microsatellite-based) genetic linkage map. This was done to facilitate efficient genome scans and comparisons among cichlid genomes. We constructed a medium-density genetic linkage map of microsatellite markers of Astatotilapia burtoni. The mapping cross was derived from two inbred laboratory lines to obtain F2 progeny by intercrossing. The map revealed 25 linkage groups spanning 1249.3 cMof the genome (size of about 950 Mb) with an average marker spacing of 6.12 cM. The seven Hox clusters, ParaHox C1, and two paralogs of Pdgfrb were - as expected - mapped to different linkage groups, thus supporting the hypothesis of a teleostspecific genome duplication. The Astatotilapia burtoni linkage map was compared to the other two available maps for cichlids using shared microsatellite markers that showed conservation and much retained synteny across chromosomes (linkage groups) among the genomes of East African cichlid that are known so far.
Projektbezogene Publikationen (Auswahl)
- 2005. Conservation and co-option in developmental programs: the importance of homology relationships. Frontiers in Zoology 2(1): 15
Sanetra, M., Becker, M.-B., Begemann, G. and A. Meyer
- 2005. Microsatellites from the burbot (Lota lota), a freshwater gadoid fish (Teleostei). Molecular Ecology Notes 5: 390-392
Sanetra, M. and A. Meyer
- 2005. Strong Reproductive Skew Among Males in the Multiply Mated Swordtail Xiphophorus multilineatus (Teleostei). Journal of Heredity 96:346–355
Luo, J., M. Sanetra, M. Schartl, and A. Meyer
- 2006. Genetic admixture of burbot (Teleostei: Lota lota) in Lake Constance from two European glacial refugia. Molecular Ecology 15: 3583- 3600
Barluenga, M., Sanetra, M. and A. Meyer
- 2007. Isolation and characterization of short tandem repeats in an invasive swimbladder nematode, parasitic in Atlantic freshwater eels, Anguillicola crassus. Molecular Ecology Notes 7: 1051-1053
Wielgoss, S., Sanetra, M., Meyer, A. and T. Wirth
- 2009. A microsatellite-based genetic linkage map of the cichlid fish, Astatotilapia burtoni (Teleosei): a comparison of genomic architectures among rapidly speciating cichlids. Genetics 182: 387–397
Sanetra, M., Henning, F., Fukamachi, S. and A. Meyer
- 2010. Genetic structure of the vairone Telestes souffia in the eastern part of Lake Constance, central Europe. Journal of Fish Biology 77: 1158-1164
Muenzel, F.M., Salzburger, W., Sanetra, M., Grabherr, B. and A. Meyer
