Strukturelle Charakterisierung des Multidrug-Transporters EmrE mittels MAS Festkörper-NMR-Spektroskopie

• Antragsteller: Professor Dr. Bernd Reif
• Fachliche Zuordnung: Biophysik
• Förderung: Förderung von 2004 bis 2009
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5424517

• Erstellungsjahr: 2010

Zusammenfassung der Projektergebnisse

Keine Zusammenfassung vorhanden

Projektbezogene Publikationen (Auswahl)

• Measurement of residual dipolar couplings in peptidic inhibitors weakly aligned by transient binding to peptide amyloid fibrils. J. Biomol. NMR 29, 525- 530 (2004)
  Chen, Z., Reif, B.
  
• Characterization of chemical exchange between soluble and aggregated states of β-amyloid by solution state NMR upon variation of the salt conditions. Biochemistry 44 (5), 1444-1452 (2005)
  Narayanan, S., Reif, B.
  
• Characterization of dynamics by MAS Solid-State NMR of uniformly deuterated proteins. J. Am. Chem. Soc. 127 (32), 11208- 11209 (2005)
  Hologne, M., Faelber, K., Diehl, A., Reif, B.
  
• Detection of dynamic water in a microcrystalline sample of the SH3 domain of alphaspectrin by MAS solid-state. J. Biomol. NMR 31, 295-310 (2005)
  Chevelkov, V., Faelber, K., Diehl, A., Heinemann, U., Oschkinat, H., Reif, B.
  
• Resolution enhancement in MAS solidstate NMR by application of 13C homonuclear scalar decoupling during acquisition. J. Magn. Reson. 172, 56-62 (2005)
  Chevelkov, V., Chen, Z., Bermel, W., Reif, B.
  
• Structure and Orientation of Peptide Inhibitors Bound to Alzheimer's disease beta-amyloid fibrils. J. Mol. Biol. 354, 760-776 (2005)
  Chen, Z., Krause, G., Reif, B.
  
• Characterization of dynamic processes using deuterium in uniformly 2H,13C,15N enriched peptides by MAS solid-state NMR. J. Magn. Reson. 178, 20-28 (2006)
  Hologne, M., Chen, Z., Reif, B.
  
• High resolution proton detected proton-carbon correlation spectra of a protein in MAS solid-state NMR spectroscopy. J. Am. Chem. Soc. 128, 12620-12621 (2006)
  Agarwal, V., Diehl, A., Skrynnikov, N., Reif, B.
  
• Protein Side-Chain Dynamics Observed by Solution- and Solid-state NMR: Comparative Analysis of Methyl 2H Relaxation Data. J. Am. Chem. Soc. 128, 12354-12355 (2006)
  Reif, B., Xue, Y., Agarwal, V., Pavlova, M. S., Hologne, M., Diehl, A., Ryabov, Y. E., Skrynnikov, N. R.
  
• The Conformation of Cyclo(−D-Pro−Ala4−) as a Model for Cyclic Pentapeptides of the DL4 Type. J. Am. Chem. Soc. 128, 13806-13814 (2006)
  Heller, M., Sukopp, M., Tsomaia, N., John, M., Mierke, D. F., Reif, B., Kessler, H.
  
• Ultra-high resolution in solid-state NMR at high levels of deuteration. Angew. Chem. Int. Edt. 45, 3878-3881 (2006)
  Chevelkov, V., Rehbein, K., Diehl, A., Reif, B.
  
• Yeast prion protein, Sup35, fibril formation proceeds by addition and substraction of oligomers. ChemBioChem. 7, 757-765 (2006)
  Narayanan, S., Walter, S., Reif, B.
  
• αB-crystalline competes with Alzheimer's disease beta-amyloid peptide for peptide-peptide interactions and induces oxidation of Abeta-Met35. FEBS Lett. 580, 5941-5946 (2006)
  Narayanan, S., Kamps, B., Boelens, W., Reif, B.
  
• Combined Analysis of 15N Relaxation Data from Solid- and Solution-State NMR Spectroscopy. J. Am. Chem. Soc. 129, 12594-12595 (2007)
  Chevelkov, V., Zhuravleva, A. V., Xue, Y., Reif, B., Skrynnikov, N. R.
  
• Differential Line Broadening in MAS solid-state NMR due to Dynamic Interference. J. Am. Chem. Soc. 129, 10195-10200 (2007)
  Chevelkov, V., Faelber, K., Schrey, A., Rehbein, K., Diehl, A., Reif, B.
  
• MAS Solid-State NMR Studies on the Multidrug Transporer EmrE. BBA - Biomembranes 1768, 3036-3043 (2007)
  Agarwal, V., Fink, U., Schuldiner, S., Reif, B.
  
• Measurement of Differential 15N-Hα/β T2 Relaxation Times in a Perdeuterated Protein by MAS Solid-State NMR. Mag. Res. Chem. 45 (S1) S156-S160 (2007)
  Chevelkov, V., Diehl, A., Reif, B.
  
• Methyl rotation barriers in proteins from 2H relaxation data. Implications for protein structure. J. Am. Chem. Soc. 129, 6827-6838 (2007)
  Xue, Y., Pavlova, M. S., Ryabov, Y. E., Reif, B., Skrynnikov, N. R.
  
• Sensitivity Enhancement Using Paramagnetic Relaxation in MAS Solid State NMR of Perdeuterated Proteins. J. Magn. Reson. 189, 209-216 (2007)
  Linser, R., Chevelkov, V., Diehl, A., Reif, B.