Characterisation of microtubule glycylation during neuronal differentiation (B08*)

• Subject Area: Biological and Biomimetic Chemistry
• Term: since 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 325871075

The so called “tubulin code” has emerged to be the key for organisation of a neural cytoskeleton and during development of nervous tissues. In the proposed project, we focus on microtubules glycylation, which is critical for cilia function and neuronal development. Only a little is known about the scope and function of glycylation and polyglycylation on microtubules and other non-tubulin proteins in neurons. We propose to develop a set of glycine probes for mass spectrometry-based chemical proteomics to uncover the scope and selectivity of (poly-)glycylation during neural differentiation and maturation. Our findings will contribute to better understanding of neurodevelopment and associated ciliopathies.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1309: Chemical Biology of Epigenetic Modifications
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Pavel Kielkowski, Ph.D.