The mechanistic and functional impact of RNA modifications on alternative splicing (B01)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

N6-methyladenosine (m6A) affects almost every stage of mRNA metabolism, including alternative splicing, and its alteration is associated with various physiological defects and diseases. Here, all m6A-dependent splicing events and all m6A sites that affect splicing will be mapped. The mechanistic interplay of effector proteins and the role of cis-regulatory elements that influence m6A-mediated regulation will be evaluated and links to RNA-binding proteins tested. This work will use Drosophila and human cells to work out conservation and differences and will be extended to the potential roles of other RNA modifications in alternative splicing regulation.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Professor Dr. Christoph Dieterich; Julian König, Ph.D.; Professor Dr. Jean-Yves Roignant