Multiple capping and decapping pathways for NAD-modified RNAs and their relation of RNA processing (A02)

• Subject Area: Biochemistry
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Modification at RNA 5’-ends with the non-canonical cap NAD+ has been described in bacteria, yeast and mammalian cell lines but the biological consequences of NAD+ capping remain poorly understood. Here, the influence of 5’-NAD+ capping on various processes in the life of an RNA, from its transcription, to RNA splicing, 3’-cleavage and polyadenylation, as well as RNA turnover will be addressed. Moreover, a possible influence of NAD+ capping on the introduction of further modifications, in particular methylations, into RNA will be assessed. Finally, the NAD+ decapping process will be investigated.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professor Dr. Andres Jäschke