confocal STimulated Emission Depletion (STED) microscope system

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 427466144

In the Anatomical Institute are currently localized microscopes equipped with epifluorescence technology only. For the scientific progress of groups from the anatomical, biochemical and physiological departments a high demand on confocal microscopes with high resolution technology exists. Such a new technology is the STED-Stimulated Emission Depletion. It permits imaging with a resolution of low as 30 nm, and belongs to the high resolution technology with increasing demand. It enables the applicants to perform fluorescence imaging in extremely high quality of many subcellular structures and proteins and permits to solve many cell biological questions. The laser configuration of the applied STED microscope enables the imaging of up to eight different fluorochromes at the same time including its exact excitation, which permits versatile applications. At the university of Kiel no super resolution microscope exists. At the same time, a high demand across departments exists not only to support existing funded projects (f. e. SFB877 “Proteolysis as a Regulatory Event in Pathophysiology”, GRK2154 “Materials 4 Brain“ and various project fundings) but also to initiate new funded projects.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Konfokales STimulated Emission Depletion (STED) Mikroskop System
• Instrumentation Group: 5090 Spezialmikroskope
• Applicant Institution: Christian-Albrechts-Universität zu Kiel
• Leader: Professorin Dr. Franziska Theilig