The analysis of DNA repair at sites of single ribonucleotide insertions (09)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 393547839

Misincorporated ribonucleotide monophosphates (rNMPs) are the most frequently occurring type of endoge-nous DNA lesion. When not repaired properly, rNMPs lead to neurological disorders and cancer. The aim of this project is to understand how and when rNMPs are repaired on a genome-wide scale. We will build on data from the first funding period to elucidate rNMP repair pathways that act independently of the canonical RNase H2 enzyme, as these represent potential targets in RNase H2-deficient cancers. Additionally, we will elucidate how RNase H2 acts in a temporal manner at rNMPs. Our results will add critical detail to under-standing how the most frequent DNA lesion is repaired to maintain a stable genome.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1361: Regulation of DNA Repair and Genome Stability
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professor Brian Luke, Ph.D.