miRNAs as systemic specificity determinants in autoregulation of symbiosis (C07*)

• Subject Area: Organismic Interactions, Chemical Ecology and Microbiomes of Plant Systems
• Term: from 2018 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 232631280

Here, we propose to investigate a candidate micro RNA (miRNA), miR171c, as possible systemic inhibitory signal (SDI) in autoregulation of nodulation. To confirm its SDI-like properties, and identify further SDI-like candidate miRNAs, we plan to perform small RNA sequencing followed by target identification using existing degradome libraries, and functionally analyze the respective miRNA-target nodes. We will further use a fluorophore-based approach to define domains of micro RNA mediated susceptibility control in roots during symbiosis initiation and progression.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1101: Molecular Encoding of Specificity in Plant Processes
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Head: Professorin Dr. Katharina Markmann