G-Protein-coupled receptors in endothelial cells: from single-cell expression to new functions (A12*)

• Subject Area: Pharmacology
• Term: from 2018 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 75732319

G-protein-coupled receptors (GPCRs) mediate the intracellular effects of a multitude of systemic and local mediators and thereby importantly modulate endothelial function. To define new roles for GPCRs in endothelial cells (EC), we performed a single-cell GPCR expression analysis in freshly isolated murine EC from different vascular beds and disease states. We identified a number of GPCRs selectively expressed in inflamed EC from brain, aorta and lung. Focusing on three orphan GPCRs that have not yet been implicated in endothelial biology, we here will analyze these orphan receptors in vitro and in tamoxifen-inducible, EC-specific knockout mice in vivo.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 834: Endothelial Signalling and Vascular Repair
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professorin Dr. Nina Wettschureck