Quantitative Interactomics to characterize lncRNPs in vitro and in vivo

• Applicant: Dr. Falk Butter
• Subject Area: Molecular Biology and Physiology of Neurons and Glial Cells
• Term: from 2017 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 389361745

The function of lncRNA is currently incompletely understood in any system, including neurons. We here propose to use our developed quantitative RNA-centered interactomics to map protein binders to diverse neuronal lncRNA within this Priority Program. Additionally, we aim to further develop this to a sensitive in vivo technique that will allow purification of lncRNPs from neuronal cells to enhance the characterization of lncRNA functionality. This approach is extendable to other RNA species such as miRNA.

• DFG Programme: Priority Programmes
• Subproject of: SPP 1738: Emerging Roles of Non-Coding RNAs in Nervous System Development, Plasticity and Disease