Combinatorial control of gene expression by heterodimers of the group C/S1 bZIP transcription factor network
Zusammenfassung der Projektergebnisse
Soil salinity increasingly causes crop losses worldwide. Although roots are the primary targets of salt stress, the signalling networks that facilitate metabolic reprogramming to induce stress tolerance in roots are less understood than those in leaves. Here, a combination of transcriptomic and metabolic approaches was performed in salt-treated Arabidopsis thaliana roots, which revealed that the group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 reprogram the primary C- and N-metabolism. In particular, gluconeogenesis and amino acid catabolism are affected by these transcription factors. Importantly, bZIP1 expression reflects the cellular stress and energy status in roots. In addition to the well-described abiotic stress response pathway initiated by the hormone Abscisic Acid (ABA) and executed by SnRK2 (Snf1-RELATED-PROTEIN-KINASE2) and AREB-like bZIP factors, we identified a structurally related ABA-independent signalling module consisting of SnRK1s and S1-bZIPs. Crosstalk between these signalling pathways recruits particular bZIP factor combinations to establish at least four distinct gene expression patterns. Class 1 comprises classical abiotic stress response genes which are regulated by AREB-like bZIPs. In contrast, genes belonging to class 3 (controlled by S1-bZIPs), class 2 (regulated by both groups) and class 4 (regulated by C/S1 heterodimers) are involved in metabolic reprogramming. Indeed, mutant analyses supported the view that via metabolic reprogramming, C/S1 bZIPs contribute to the plant’s tolerance against salt stress. Understanding this signalling network provides a framework for securing future crop productivity.
Projektbezogene Publikationen (Auswahl)
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(2009) Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and development. Plant Mol Biol. 69, 107-19
Weltmeier, F., Rahmani, F., Ehlert, A., Dietrich, K., Schütze, K., Wang, X., Chaban, C., Hanson, J., Teige, M., Harter, K., Vicente-Carbajosa, J., Smeekens, S., Dröge-Laser, W.
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(2009). A pivotal role of the basic leucine zipper transcription factor bZIP53 in the regulation of Arabidopsis seed maturation gene expression based on heterodimerization and protein complex formation. Plant Cell, 21, 1747-1761
Alonso R, Onate-Sanchez L, Weltmeier F, Ehlert A, Diaz I, Dietrich K, Vicente-Carbajosa J, Dröge-Laser W
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(2011) The sucrose-regulated Arabidopsis transcription factor bZIP11 reprograms metabolism and regulates trehalose metabolism. New Phytol. 191: 733-45
Ma J, Hanssen M, Lundgren K, Hernández L, Delatte T, Ehlert A, Liu CM, Schluepmann H, Dröge-Laser W, Moritz T, Smeekens S, Hanson J
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(2011). Heterodimers of the Arabidopsis transcription factors bZIP1 and bZIP53 reprogram amino acid metabolism during low energy stress. Plant Cell 23: 381-395
Dietrich K, Weltmeier F, Ehlert A, Weiste C, Stahl M, Harter K, Dröge-Laser W
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(2012) Bioinformatic cis-element analyses performed in Arabidopsis and rice disclose bZIP- and MYB-related binding sites as potential AuxRE-coupling elements in auxin-mediated transcription. BMC Plant Biology 12:125
Berendzen KW, Weiste C, Wanke D, Kilian J, Harter K, Dröge-Laser W
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(2015) SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants. eLife
Mair A, Pedrotti L, Wurzinger, B, Anrather D, Simeunovic A, Weiste C, Valerio C, Dietrich K, Kirchler T, Nägele T, Vicente Carbajosa J, Hanson J, Baena-González E, Chaban C, Weckwerth W, Dröge-Laser W, Teige M
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(2015). Crosstalk between Two bZIP Signaling Pathways Orchestrates Salt-Induced Metabolic Reprogramming in Arabidopsis Roots. Plant Cell tpc.15.00163
Hartmann, L, Pedrotti, L, Weiste, C, Fekete, A, Schierstaedt, J, Göttler, J, Kempa, S, Krischke, M, Dietrich, K, Mueller, MJ, Vicente-Carbajosa, J, Hanson, J, Dröge-Laser, W
