Project Details
Dielectrophoresis-assisted Raman micro-spectroscopy for pathogen identification
Applicant
Dr. Eva-Maria Laux
Subject Area
Medical Physics, Biomedical Technology
Analytical Chemistry
Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry
Analytical Chemistry
Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry
Term
Funded in 2016
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 323403781
Biosensors are important diagnostic devices that measure specific analytes, e.g. biomarkers, that help to identify and monitor diseases. They are further used in environmental studies to quantify analytes for quality assurance of, e.g., food or drinking water. The goal of this research project is to combine dielectrophoresis, a fast and selective method for analyte pre-concentration or immobilization, with Raman spectroscopy, for the sensitive and specific detection of pathogens like bacteria or viral particles on a flexible antibody-functionalized platform.Antibodies will be immobilized by application of AC electric fields on nanoelectrodes; subsequently, pathogens will selectively be captured by the antibodies, and the pathogens will be identified by Raman spectroscopy. The use of nanoelectrode arrays with several thousands electrodes offers rapid analyses of large numbers of individual pathogens. Moreover, several different pathogens can be immobilized on one chip for multiplexed analyses. This approach is very promising for the fast and efficient detection of pathogens and, hence, for the field of biosensor research.
DFG Programme
Research Fellowships
International Connection
Canada