Project Details
Projekt
Conformational switching of Bcl2 proteins and their complexes in a native membrane environment (B13)
Subject Area
Biochemistry
Term
from 2016 to 2024
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 201302640
In project B13, we will investigate the structural transformation of the pro-apoptotic Bcl2 protein Bak taking place on a membrane surface, a process that is required for downstream pore formation, and will probe its interaction with anti-apoptotic BclxL in a full-length context and in a native phospholipid bilayer environment. Furthermore, we will perform a comparative biophysical and structural analysis of pro- and anti-apoptotic Bcl2 proteins to be able to extract the hallmarks defining a pro-apopototic member. In addition, we will continue working on the high-resolution structure determination and functional characterisation of the VDAC ion channel in phospholipid nanodiscs and its complex with anti-apopotitc BclxL. For reaching these project goals, we will mainly use NMR spectroscopy, as well as a large variety of other structural and biophysical methods that are available in the CRC1035.
DFG Programme
Collaborative Research Centres
Applicant Institution
Technische Universität München (TUM)
Project Head
Professor Dr. Franz Hagn
