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Molecular Mechanisms underlying selection of cargo, scission, and coat release of intracellular vesicular carriers

Subject Area Biochemistry
Term from 2016 to 2019
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 287901311
 
Final Report Year 2020

Final Report Abstract

In the project “Molecular Mechanisms underlying selection of cargo, scission, and coat release of intracellular vesicular carriers” we have investigated i) the structural basis for the COPI scission reaction mediated by Arf, and whether a general mechanism exists for membrane scission catalyzed by GTP-binding proteins, ii) the selectivity of different COPI and COPII coat protein isoforms for the uptake of protein cargo by analysing the proteomes of isotypic vesicles reconstituted with recombinant coat proteins from semi intact cells, and iii) how GTPase-activating proteins catalyze the removal of the COPI coat and possibly aid the conversion of polymerized coat proteins to their soluble form.

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