Molekulare Mechanismen der Frachtaufnahme, Abschnürung und der Dissoziation der Hülle von intrazellulären Transportvesikeln

• Antragsteller: Professor Dr. Felix Wilhelm Theodor Wieland
• Fachliche Zuordnung: Biochemie
• Förderung: Förderung von 2016 bis 2019
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 287901311

• Erstellungsjahr: 2020

Zusammenfassung der Projektergebnisse

In the project “Molecular Mechanisms underlying selection of cargo, scission, and coat release of intracellular vesicular carriers” we have investigated i) the structural basis for the COPI scission reaction mediated by Arf, and whether a general mechanism exists for membrane scission catalyzed by GTP-binding proteins, ii) the selectivity of different COPI and COPII coat protein isoforms for the uptake of protein cargo by analysing the proteomes of isotypic vesicles reconstituted with recombinant coat proteins from semi intact cells, and iii) how GTPase-activating proteins catalyze the removal of the COPI coat and possibly aid the conversion of polymerized coat proteins to their soluble form.

Projektbezogene Publikationen (Auswahl)

• (2017) 9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments. Elife 16;6, pii: e26691
  Dodonova S.O., Aderhold P., Kopp J., Ganeva I, Röhling S, Hagen WJH, Sinning I, Wieland F, Briggs JAG.
  
• (2018) Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Ann.Rev. Biophys 47
  Béthune J and Wieland FT
  
• (2019) Proteomic Profiling of COPII and COPI Vesicles. Cell Reports 26, 250-265
  Adolf F, Rhiel M, Hesssling B, Gao Q, Hellweg A, Bethune J, Wieland FT