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Understanding the impact of ovarian stimulation on oocyte and embryo quality by tandem RNA and protein expression analysis of oocytes and preimplantation embryonic stages

Subject Area Reproductive Medicine, Urology
Term from 2016 to 2020
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 279728933
 
Final Report Year 2021

Final Report Abstract

Using the paradigm of gonadotropin-induced superovulation, co-PIs Leila Taher, Georg Fuellen and Michele Boiani applied mass spectrometry, RNA sequencing and bioinformatic analysis to study the impact on oocyte and embryo quality of a common treatment used in medically- assisted reproduction, in the mouse model of human reproduction. Our published and submitted work showed that 1), in the vast majority of cases, transcript profiles offer no reliable information about protein profiles; 2) protein abundances can be expressed using the riBAQ algorithm, which returns values in the vicinity of picomoles, thereby enabling an educated and accurate targeting of gene product for destruction directly at the protein level (e.g. ‘Trim- away’); 3) the superovulation changes the abundance of specific proteins in oocytes and embryos, without any significant change in the transcriptome. The third and last finding was the most surprising, because it could not have been predicted otherwise. Who knows how many other false negatives there are to be uncovered in the field of the treatments used in medically- assisted reproduction (i.e. treatments regarded to as free of side effects according to transcript analysis, while in fact those effects are there, just in another stage of the gene expression cascade). Together, these findings make us aware that a potential threat for oocyte quality has been underestimated when the sole transcriptome is examined, and that superovulated oocytes are clearly distinguishable from – and probably inferior to - naturally ovulated oocytes, in terms of protein composition. Thus, conclusions about oocyte quality in the context of ovarian stimulation (human) and superovulation (mice) might be not as safe as we had assumed, and derivative embryos might not support conclusions that universally apply for natural development. The progress reported here would not have been possible without the development of suitable bioinformatic pipelines, delivered by co-PIs Leila Taher and Georg Fuellen, which disentangled the complexity of the biological data of co-PI Michele Boiani.

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