2-Photonen-Mikroskop
Final Report Abstract
The Sigrist group used the instrument to in vivo analyze Ca2+ signals triggered by neuronal activity and used fluorescence-based sensors to directly measure release of synaptic vesicle in intact Drosophilae also in response to natural odors. They occupy the instrument for about 1-2 days the week. They used these approaches in two major projects: 1. Characterization of synpase functional organization during aging, and ways to protect synapse organization in aging animals. 2. Characterization of the role of distinct synaptic "release modules" for information processing in the Drosophila brain. They here found that a burst sensor release machinery equipped synapses fulfills different computing tasks than spare sensing synapses when analyzing the olfactory system of Drosophila. The Hiesinger group performs long-term developmental imaging sessions of 24 hours at a time 3-4 days a week. Two types of developmental imaging experiments are performed: 1. intravital imaging of brain development in intact developing Drosophila pupae; 2. ex vivo brain culture imaging on normally developing fly brain. Specifically, we have shown that the wiring of Drosophila photoreceptors to the brain is independent of the developmental dynamics of their postsynaptic target cells. In a second recent major study we have obtained large 4D datasets of the filopodial dynamics of photoreceptors in the brain. Together with scientists from the Zuse Institute Berlin as well as colleagues from the mathematics department at FU Berlin, we have developed novel tools for the quantification of filopodial dynamics and stochastic models for robust synapse formation based on stochastif filopodial dynamics. Thirdly, we have utilized the Leica MP-OPO-MPX for live imaging studies of intracellular endosomal dynamics and membrane protein degradation at axon terminals in the living brain.
Publications
- Inhibition of oxidative stress in cholinergic projection neurons fully rescues aging-associated olfactory circuit degeneration in Drosophila. eLife 2018;7:e32018
Hussain, Pooryasin, ... Sigrist, ...
(See online at https://doi.org/10.7554/eLife.32018) - Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol. 2018 Apr 2;28(7):1027-1038.e4
Jin, ... Hiesinger
(See online at https://doi.org/10.1016/j.cub.2018.02.032)
