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Projekt Druckansicht

Erstellung einer Chromosomenkarte für Spirodela polyrhiza, Chromosomenhomöologie und Karyotypevolution bei den aquatischen, weitgehend asexuellen Lemnoideae, einer basalen Monocot-Gruppe von potenzieller ökonomischer Bedeutung

Fachliche Zuordnung Genetik und Genomik der Pflanzen
Förderung Förderung von 2014 bis 2021
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 256417479
 
Erstellungsjahr 2020

Zusammenfassung der Projektergebnisse

The aim of the project was to study cytological features and the composition, diversity and evolution of genomes, karyotypes of selected species of the five aquatic monocot genera of duckweeds, which are of growing economic and scientific interest, but little studied under these aspects. The main achievements of the project are: Epigenetic characterization of eu- and heterochromatic features and determination of chromosome number, genome size, rDNA loci number and nuclear and cell volumes (of guard cells) for species of all five genera. The heterochromatin distribution of these neotenic organisms remembered this of very young Arabidopsis seedlings, independent of genome size. Nuclear and cell volumes correlate positively with genome size, but negatively with phylogenetic age and frond size, while chromosome and rDNA loci number do not correlate with genome size. No deviating chromosome numbers were found for clones of the same species; chromosome numbers and genome size gave no unambiguous hint on recent natural whole genome duplications. Genome assemblies from various sequencing technologies were validated, corrected and integrated by serial mcFISH with single copy BACs into the 20 chromosomes of Spirodela polyrhiza. Combining several independent approaches yielded a robust chromosome scale genome map and revealed no gross karyotypic alterations between 7 asexual S. polyrhiza clones of different geographic origin. Homeology and evolutionary chromosome rearrangements between the only two species of the basic duckweed genus Spirodela have been elucidated and a cytogenomic map for two clones of the second species, S. intermedia, has been established. The limits of resolution of cross-FISH with BAC and unique oligo probe samples for elucidation of chromosome homeology and the routes of karyotype evolution across the duckweed genera were determined. Contributions were made to support the generation of a genome map for Landoltia punctata, the only species of the 2nd duckweed genus, and for taxonomic studies resulting in a reduction of Lemna species from 13 to 12 by our collaboration partners.

Projektbezogene Publikationen (Auswahl)

 
 

Zusatzinformationen

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