Nucleic-Acid Based Super-Resolution Microscopy

• Applicant: Professor Dr. Ralf Jungmann
• Subject Area: Biophysics
• Term: from 2014 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 251122678

• Final Report Year: 2021

Final Report Abstract

In conclusion, the research carried out through the Emmy Noether Grant has help tremendously to push DNA-based super-resolution microscopy to the next level, paving the way to quantitative high-throughput studies under biomedically relevant conditions. While we were able to push spatial resolution to below 5 nm as well as improve the labeling probes and image acquisition speed, there is still a dire need in carefully benchmarked, improved labeling probes for next-generation super-resolution microscopy, an area where we actively develop new approaches at the moment. Furthermore, we are pursuing applications of improved DNA-PAINT microscopy to cell-biological and biomedical questions such as the multiplexed visualization of cell surface receptor patterns with single protein resolution.

Publications

• Fast, background-free DNA-PAINT imaging using FRET-based probes Nano Letters (2017). 17(10): 6428-6434
  Auer, M.T. Strauss, T. Schlichthaerle, R. Jungmann
  (See online at https://doi.org/10.1021/acs.nanolett.7b03425)
• Multiplexed 3D super-resolution imaging of whole cells using Spinning Disk Confocal Microscopy and DNA- PAINT Nature Communications (2017). 8: 2090
  F. Schueder, J. Lara-Guitérrez, B.J. Beliveau, S.K. Saka, H.M. Sasaki, J.B. Woehrstein, M.T. Strauss, H. Grabmayr, P. Yin, R. Jungmann
  (See online at https://doi.org/10.1038/s41467-017-02028-8)
• Super-Resolution Microscopy with DNA-PAINT Nature Protocols (2017). 12: 1198-1228
  J. Schnitzbauer, M.T. Strauss, T. Schlichthaerle, F. Schueder, R. Jungmann
  (See online at https://doi.org/10.1038/nprot.2017.024)
• Universal Super-Resolution Multiplexing by DNA Exchange Angewandte Chemie Int. Ed. (2017). 56: 4052-4055
  F. Schueder, M.T. Strauss, D. Hoerl, J. Schnitzbauer, T. Schlichthaerle, S. Strauss, P. Yin, H. Harz, H. Leonhardt, R. Jungmann
  (See online at https://doi.org/10.1002/anie.201611729)
• Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging Nature Methods (2018). 15: 685–688
  S. Strauss, P.C. Nickels, M.T. Strauss, V.J. Sabinina, J. Ellenberg, J.D. Carter, S. Gupta, N. Janjic, R. Jungmann
  (See online at https://doi.org/10.1038/s41592-018-0105-0)
• Quantifying absolute addressability in DNA origami with molecular resolution Nature Communications (2018). 9: 1600
  M.T. Strauss, F. Schueder, D. Haas, P.C. Nickels, R. Jungmann
  (See online at https://doi.org/10.1038/s41467-018-04031-z)
• Site-specific labeling of Affimers for DNA-PAINT microscopy Angewandte Chemie Int. Ed. (2018). 57: 11060-11063
  T. Schlichthaerle, A.S. Eklund, F. Schueder, M.T. Strauss, C. Tiede, A. Curd, J. Ries, M. Peckham, D.C. Tomlinson, R. Jungmann
  (See online at https://doi.org/10.1002/anie.201804020)
• 124-color super-resolution imaging by engineering DNA-PAINT blinking kinetics Nano Letters (2019). 19(4): 2641-2646
  O.K. Wade, J.B. Woehrstein, P.C. Nickels, S. Strauss, F. Stehr, J. Stein, F. Schueder, M.T. Strauss, M. Ganji, J. Schnitzbauer, H. Grabmayr, P. Yin, P. Schwille, R. Jungmann
  (See online at https://doi.org/10.1021/acs.nanolett.9b00508)
• An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions Nature Methods (2019). 16: 1101-1104
  F. Schueder, J. Stein, F. Stehr, A. Auer, B. Sperl, M.T. Strauss, P. Schwille, R. Jungmann
  (See online at https://doi.org/10.1038/s41592-019-0584-7)
• Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA- PAINT Angewandte Chemie Int. Ed. (2019). 58: 13004-13008
  T. Schlichthaerle, M.T. Strauss, F. Schueder, A. Auer, B. Nijmeijer, M. Kueblbeck, V.J. Sabinina, J.V. Thevathasan, J. Ries, J. Ellenberg, R. Jungmann
  (See online at https://doi.org/10.1002/anie.201905685)
• Peptide-PAINT super-resolution imaging using transient coiled coil interactions Nano Letters (2020). 20(09): 6732-6737
  A.S. Eklund, M. Ganji, G. Gavins, O. Seitz, R. Jungmann
  (See online at https://doi.org/10.1021/acs.nanolett.0c02620)
• Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT Nature Methods (2020). 17: 78-791
  S. Strauss, R. Jungmann
  (See online at https://doi.org/10.1038/s41592-020-0869-x)