Nukleinsäurebasierte Super-Auflösungs-Mikroskopie

• Antragsteller: Professor Dr. Ralf Jungmann
• Fachliche Zuordnung: Biophysik
• Förderung: Förderung von 2014 bis 2019
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 251122678

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

In conclusion, the research carried out through the Emmy Noether Grant has help tremendously to push DNA-based super-resolution microscopy to the next level, paving the way to quantitative high-throughput studies under biomedically relevant conditions. While we were able to push spatial resolution to below 5 nm as well as improve the labeling probes and image acquisition speed, there is still a dire need in carefully benchmarked, improved labeling probes for next-generation super-resolution microscopy, an area where we actively develop new approaches at the moment. Furthermore, we are pursuing applications of improved DNA-PAINT microscopy to cell-biological and biomedical questions such as the multiplexed visualization of cell surface receptor patterns with single protein resolution.

Projektbezogene Publikationen (Auswahl)

• Fast, background-free DNA-PAINT imaging using FRET-based probes Nano Letters (2017). 17(10): 6428-6434
  Auer, M.T. Strauss, T. Schlichthaerle, R. Jungmann
  
• Multiplexed 3D super-resolution imaging of whole cells using Spinning Disk Confocal Microscopy and DNA- PAINT Nature Communications (2017). 8: 2090
  F. Schueder, J. Lara-Guitérrez, B.J. Beliveau, S.K. Saka, H.M. Sasaki, J.B. Woehrstein, M.T. Strauss, H. Grabmayr, P. Yin, R. Jungmann
  
• Super-Resolution Microscopy with DNA-PAINT Nature Protocols (2017). 12: 1198-1228
  J. Schnitzbauer, M.T. Strauss, T. Schlichthaerle, F. Schueder, R. Jungmann
  
• Universal Super-Resolution Multiplexing by DNA Exchange Angewandte Chemie Int. Ed. (2017). 56: 4052-4055
  F. Schueder, M.T. Strauss, D. Hoerl, J. Schnitzbauer, T. Schlichthaerle, S. Strauss, P. Yin, H. Harz, H. Leonhardt, R. Jungmann
  
• Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging Nature Methods (2018). 15: 685–688
  S. Strauss, P.C. Nickels, M.T. Strauss, V.J. Sabinina, J. Ellenberg, J.D. Carter, S. Gupta, N. Janjic, R. Jungmann
  
• Quantifying absolute addressability in DNA origami with molecular resolution Nature Communications (2018). 9: 1600
  M.T. Strauss, F. Schueder, D. Haas, P.C. Nickels, R. Jungmann
  
• Site-specific labeling of Affimers for DNA-PAINT microscopy Angewandte Chemie Int. Ed. (2018). 57: 11060-11063
  T. Schlichthaerle, A.S. Eklund, F. Schueder, M.T. Strauss, C. Tiede, A. Curd, J. Ries, M. Peckham, D.C. Tomlinson, R. Jungmann
  
• 124-color super-resolution imaging by engineering DNA-PAINT blinking kinetics Nano Letters (2019). 19(4): 2641-2646
  O.K. Wade, J.B. Woehrstein, P.C. Nickels, S. Strauss, F. Stehr, J. Stein, F. Schueder, M.T. Strauss, M. Ganji, J. Schnitzbauer, H. Grabmayr, P. Yin, P. Schwille, R. Jungmann
  
• An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions Nature Methods (2019). 16: 1101-1104
  F. Schueder, J. Stein, F. Stehr, A. Auer, B. Sperl, M.T. Strauss, P. Schwille, R. Jungmann
  
• Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA- PAINT Angewandte Chemie Int. Ed. (2019). 58: 13004-13008
  T. Schlichthaerle, M.T. Strauss, F. Schueder, A. Auer, B. Nijmeijer, M. Kueblbeck, V.J. Sabinina, J.V. Thevathasan, J. Ries, J. Ellenberg, R. Jungmann
  
• Peptide-PAINT super-resolution imaging using transient coiled coil interactions Nano Letters (2020). 20(09): 6732-6737
  A.S. Eklund, M. Ganji, G. Gavins, O. Seitz, R. Jungmann
  
• Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT Nature Methods (2020). 17: 78-791
  S. Strauss, R. Jungmann