Optische Eindämmung epileptischer Anfälle mittels Zwei-Photonen-Licht
Kognitive und systemische Humanneurowissenschaften
Molekulare Biologie und Physiologie von Nerven- und Gliazellen
Zusammenfassung der Projektergebnisse
This project combined electrophysiology with fast resonant two-photon calcium imaging in a mouse model of acute pharmacological seizures allowing the study of spatiotemporal population activity patterns during ictogenesis and cortical spread of epileptic discharges, with high temporal fidelity, neural subtype identifiability and single cell resolution in vivo. One main goal of this project was to investigate shared and differential patterns of epileptic network dynamics within the epileptic focus and neighboring cortical territories. Neural recruitment in either area, and across the cortical column, displayed consistent spatio-temporo-progressive motifs during recurrent ictal events. This reliability was found despite great temporal network stretchability consistent with a neural mesh where ictal progression may vary in time but cannot betray current neural connectivity. Current efforts involve the study and optogenetic activity modulation of various interneuronal sub-populations during ictal activity in vivo. Together, this project intensifies the conceptual understanding of epileptic networks in the intact brain at finest spatiotemporal scale. The study of reliable ictal network motifs and the roles of neural subtypes therein will help identifying novel therapeutic targets for more efficient seizure control.
Projektbezogene Publikationen (Auswahl)
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Reliable and Elastic Propagation of Cortical Seizures In Vivo. Cell Reports Volume 19, Issue 13, 27 June 2017, Pages 2681-2693
Michael Wenzel, Jordan P. Hamm, Darcy S. Peterka and Rafael Yuste
