Quantitative protein analysis (Z1)

• Subject Area: Biology
• Term: from 2012 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 201348542

We will continue to support all research projects with mass spectrometry (MS) expertise according to their needs; for example the Knob lab (TP10) and the Hell/Wirtz lab (TP13) with the analysis of protein N-termini, the Strahl lab (TP11) with identification of mannosylated peptides, the Pereira lab (TP21) with phospho-proteomics and the Sinning lab (TP22) with X-link MS. In addition, we will establish/improve procedures of common interest: 1. Generation of a “general” SILAC spike-in standard. 2. Extending shotgun proteomics by targeted protein quantification. 3. A toolbox to facilitate data interpretation of proteomics data sets.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1036: Cellular Surveillance and Damage Response
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Heads: Professor Dr. Matthias Peter Mayer, until 6/2016; Dr. Thomas Ruppert