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Projekt Druckansicht

Switchable polymer interfaces for bottom-up stimulation of mamalian cells

Fachliche Zuordnung Experimentelle und Theoretische Polymerphysik
Experimentelle Physik der kondensierten Materie
Förderung Förderung von 2012 bis 2016
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 200926410
 
Erstellungsjahr 2015

Zusammenfassung der Projektergebnisse

As a main result of the project we were able to successfully design and characterize a responsive thin polymer film platform which comprised of functionalized polymer brushes for the stimuli-triggered exposure of functional molecules to the bioengineered interfaces of cells. We could demonstrate stimulation of cells using this switchable polymer brush platform and present an approach for the functionalization of brush systems with molecules typically used to stimulate cellular responses and developed approaches to design switchable bio-interfaces for guided cell behavior. We determined in particular that by stimulation with temperature and pH local changes in the brush-bio-interface are induced and can result in exposure of functional molecules to the surface. Dedicated techniques like in-situ IR-ellipsometry as well as computer simulations were employed to determine details of the switching mechanism. We studied exposure kinetics and equilibrium using a range of probe techniques and compared results with coarse-grained theoretical modeling. Interactions and stimulation of human hepatoma cells and mouse embryonic stem cells with these switchable surfaces have also been investigated where the influence of functionalization has clear effect. Those results could only be obtained by the joint effort of participating groups in US and Germany bringing together their supplementary expertise.

Projektbezogene Publikationen (Auswahl)

 
 

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