Detailseite
Projekt
Pasteurella multocida Toxin als Werkzeug zur Untersuchung der Knochen- und Immunzelldifferenzierung
Antragstellerin
Professorin Dr. Katharina Hieke-Kubatzky
Fachliche Zuordnung
Immunologie
Rheumatologie
Rheumatologie
Förderung
Förderung von 2010 bis 2017
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 169332709
The project will investigate the connection between B-cell and osteoclast differentiation, using the bacterial toxin from Pasteurella multocida (PMT) as a model system. PMT is a potent mitogen with known osteoclastic properties that stimulates differentiation of bone marrow cells into osteoclasts and inhibits osteoblast function, respectively. The aim of the project is to further elucidate the mechanism of PMTinduced osteoclast differentiation and to define the role of B-cells in that process. We aim to characterise the PMT-generated B-cell and osteoclast populations and their interaction or dependence of each other. We will investigate the mechanism of that interaction on a molecular level by characterising the secreted factors and by comparing the secretome and proteome of PMT and IL7, M-CSF/RANKL or LPS derived osteoclasts. In addition, we aim to define the signalling networks involved in the interplay between B-cells and osteoclasts, with a focus on anti-apoptotic signalling through Akt and Pim kinases and the importance of migration and adhesion to further expand our understanding of the regulation of bone destruction by immunological processes.
DFG-Verfahren
Schwerpunktprogramme
Teilprojekt zu
SPP 1468:
Osteoimmunology - IMMUNOBONE - A Program to Unravel the Mutual Interactions between the Immune System and Bone
Beteiligte Personen
Professor Dr. Thomas Dörner; Dr. Annette Garbe; Professor Dr. Rudolf Manz
