Immobilization of polysialic acid on the surfaces of biometerials

• Applicant: Professor Dr. Peter Behrens (†)
• Subject Area: Biological and Biomimetic Chemistry
• Term: from 2008 to 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5471076

• Final Report Year: 2014

Final Report Abstract

The aim of WP 2.4 was to immobilize polysialic acid on the surfaces of biomaterials in order to provide materials for the construction of scaffolds for biomedical purposes, especially nerve regeneration. WP 2.4 succeeded in synthesizing nanoporous silica nanoparticles (NPSNPs) and in their functionalization, most importantly with polySia itself, but also with a growth factor and with fluorescent entities (fluorescent nanoparticles, fluorescent dyes) for imaging. Cytocompatibility testing accompanied the preparative work. WP 2.4 also succeeded in the fabrication of functionalized collagen tubes to be used as nerve guidance channels. These consisted of rolled collagen sheets decorated with NPSNPs carrying polySia. Regarding the high degrees of functionalization, both of the nanoparticles with polySia and of the collagen sheets with nanoparticles, it can be presumed that these constructs exhibit only polySia strands on their inner surface. They should thus offer a favourable milieu for nerve regeneration. As all kinds of tests performed on these scaffolds (cytocompatibility, mechanical and chemical stability) gave positive results, these are currently being tested as nerve guidance channels. In addition, it was possible to obtain semicrystalline polySia. With structural modelling, it was possible to propose a satisfactory, but not yet sufficiently substantiated model for the structure of crystalline polySia. In the course of studies, WP 2.4 interacted with nearly all the other work packages.

Publications

• Auf dem Weg zu neuartigen Biomaterialien für die Nervenregeneration: Immobilisierung von Polysialinsäure. Dissertation, Leibniz Universität Hannover 2009
  S. Steinhaus
  
• Enabling Mesoporous Materials for Click Chemistry. 21. Deutsche Zeolith- Tagung, 04.-06.03.2009, Kiel
  I. Bremer, P. Behrens
  
• Immobilization of polysialic acid on silica particles. Jahrestagung der Deutschen Gesellschaft für Biomaterialien, Tübingen, 8.-10. Oktober 2009
  S. Williams, S. Steinhaus, Y. Su, G. Dräger, A. Kirschning, P. Behrens
  
• (2010) Polysialic acid immobilized on silanized glass surfaces: a test case for its use as a biomaterial for nerve regeneration. J. Mater. Sci. Mater. Med., 21, 1371-1378
  Steinhaus, S., Stark, Y., Bruns, S., Haile, Y., Scheper, T., Grothe, C., and Behrens, P.
  (See online at https://doi.org/10.1007/s10856-009-3981-0)
• Immobilization of polysialic acid on nanoporous silica nanoparticles. Jahrestagung der Deutschen Gesellschaft für Biomaterialien, Heilbad Heiligenstadt, 18.-20. November 2010
  S. Williams, I. Bremer, A. Christel, S. Böhm, C. Kasper, T. Scheper, R. Gerardy-Schahn, P. Behrens
  
• Immobilization of polysialic acid on nanoporous silica particles. Sialoglyco 2010, Potsdam, 21.-26. August 2010
  S. Williams, I. Bremer, A. Christel, S. Böhm, C. Kasper, Y. Su, G. Dräger, A. Kirschning, R. Gerardy-Schahn, P. Behrens
  
• Nanoparticles for multiple functionalization of scaffolds for stem cell engineering. BioStar 2010, Stuttgart, 13.-15. Oktober 2010
  A. Christel, N. Ehlert, S. Williams, A. Hoffmann, C. Kasper, P. Behrens
  
• Nanoparticles for multiple functionalization of scaffolds for tissue engineering applications. Hybrid Materials, Strasbourg, 6.-10. März 2011
  A. Christel, A. Neumann, S. Williams, A. Hoffmann, C. Kasper, P. Behrens
  
• Nanoparticles for multiple functionalization of scaffolds for tissue engineering applications. World Congress on Regenerative Medicine, Leipzig, 2.-4. November 2011
  A. Christel, A. Neumann, S. Williams, A. Hoffmann, C. Kasper, P. Behrens
  
• Nanoporous silica nanoparticles: Strategies for functionalization and cell culture tests. Jahrestagung der Deutschen Gesellschaft für Biomaterialien, Gießen, 10.-12. November 2011
  S. Williams, A. Christel, A. Neumann, S. Böhm, C. Kasper, P. Behrens
  
• Multifunctionalization of nanoporous silica nanoparticles for tissue engineering applications. Nanomaterials for Biomedical Technologies, Frankfurt a. M., 6.-7. März 2012
  A. Christel, A. Neumann, S. Williams, A. Hoffmann, C. Kasper, P. Behrens
  
• Nanoporous silica nanoparticles: Strategies for functionalization and cell culture tests. Nanomaterials for Biomedical Technologies, Frankfurt a. M., 6.-7. März 2012
  S. Williams, A. Christel, A. Neumann, S. Böhm, C. Kasper, P. Behrens
  
• (2013) BMP2-loaded nanoporous silica nanoparticles promote osteogenic differentiation of human mesenchymal stem cells. RSC Adv. 3, 24222 – 24230
  Neumann, A., Christel, A., Kasper, C., and Behrens, P.
  (See online at https://doi.org/10.1039/C3RA44734K)
• Entwicklung multifunktional modifizierter Silica-Nanopartikel und deren Kompositmaterialien für Anwendungen in der Nervenregeneration. Dissertation, Leibniz Universität Hannover 2013
  S. Williams
  
• Functionalization of nanoporous silica nanoparticles for variable applications in nanomedicine. Dissertation, Leibniz Universität Hannover 2013
  A. Christel
  
• Stem cell differentiation induced by BMP2 loaded nanoporous silica nanoparticles. Topical Workshop of the Priority Program “Porous Metal- Organic Frameworks” of the DFG: MOFs in Biological and Medical Applications. Hannover, June 6-7, 2013
  A. Christel, A. Neumann, C. Kasper, P. Behrens