2-Cys Prx and PrxIIE as redox sensors, signal integrators and transmitters

• Applicants: Professor Dr. Karl-Josef Dietz; Privatdozent Dr. Hans-Peter Mock; Dr. Mark Aurel Schöttler
• Subject Area: Plant Physiology
• Term: from 2008 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 81631128

Peroxiredoxins (Prx) are ubiquitously occurring redox proteins abundantly present in different cell compartments that serve as antioxidants and are implicated in a complex signaling function. The project will elucidate the function of the chloroplast peroxiredoxins 2-Cys Prx and PrxllE as regulatory hubs detecting and transmitting redox information to diverse target functions. The project will combine (1) detailed in vitro biochemical and (2) in situ protein analyses, (3) transgenic approaches for validation, (4) a functional differentiation of both signaling hubs and (5) will lay ground for regulatory state prediction and lateron for modelling. On the one hand novel regulatory features such as the inhibitory 2-Cys Prx/nucleic acid and the activating 2-Cys Prx/fructose-1,6-phosphatase interactions will be studied on a quantitative basis, the former 2-Cys Prx/DNA interference will be compared among plant, cyanobacteria and human 2-Cys Prx (Prdxl, PrdxS). The comprehensive analysis of interactions and activity will be linked to the posttranslational state in vivo to predict the regulatory role of the 2-Cys Prx under divergent environmental conditions. Likewise, regulation of the PrxllE activation state will be analyzed in dependence of redox and reactive nitrogen species (NO, peroxinitrite) in order to assess the proposed function of Prx in the crosstalk between reactive oxygen and reactive nitrogen dependent signaling in cell acclimation.

• DFG Programme: Research Grants
• Major Instrumentation: Proteinchromatographieanlage
• Instrumentation Group: 1350 Flüssigkeits-Chromatographen (außer Aminosäureanalysatoren 317), Ionenaustauscher
• Participating Person: Professor Dr. Bernhard Grimm