Project Details
Projekt
Function and mechanism of the lysosomal polypeptide transporter ABCB9 (P09)
Subject Area
Biochemistry
Term
from 2008 to 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 57566863
ABCB9 forms a homodimeric complex, which can functionally be divided into the core complex and the N-terminal, transmembrane domainTMD0, responsible for lysosomal localization and interaction with LAMP-1/2. We will determine the molecular structure by NMR spectroscopy. Further, the interface between TMD0 and its interaction partners will be investigated. With cell biological methods the machinery for lysosomal trafficking of ABCB9 will be studied. Peptide binding and release as well as ATP hydrolysis will be analyzed by single molecule techniques and fast kinetics.
DFG Programme
Collaborative Research Centres
Applicant Institution
Goethe-Universität Frankfurt am Main
Project Head
Privatdozent Dr. Rupert Abele
