Metallnanopartikel als nichtlinear optische Antennen in photoaktiven 3D-Polymermatrizen: MNPs in Polymersomen und biomedizinische Anwendungsmöglichkeiten

• Applicants: Professor Dr. Lukas M. Eng; Professorin Brigitte Voit, Ph.D.
• Subject Area: Production Automation and Assembly Technology
• Term: from 2009 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 79520886

• Final Report Year: 2016

Final Report Abstract

In this project, we presented the photoactive polymersome formation as well as fixation of them onto solid substrates to finally trigger the nonlinear optical-induced photochemical reactions by IR light. As known, IR light is safe to biological compounds and can penetrate deeply to organic materials which are outstanding advantages for biomedical applications. In the light of what we have accomplished in this study, we believe that the established smart nanovesicles are useful for targeted and selective recognition systems especially in the field of microsystem devices and synthetic biology. The main findings of this work are summarized as follows.  Functional amphiphilic block copolymers containing azide and adamantane groups were synthesized for polymersome formation. Surface functionalization of polymersomes with NVOC groups as photoactive moieties were achieved. Herein, additional proof studies using polymersomes without cross-linker groups was performed to ensure the covalent attachment of NVOC groups.  Further photochemical reactions by UV trigger were realized to cleave the NVOC groups for liberated amine functionalities on the surface of the polymersomes. Sequential post-modification of polymersomes was achieved which proved the presence of accessible functional groups on the polymersome periphery.  The approach for polymersome immobilization allowed to creating half-dome-shaped polymersome compartments at the substrate, which are robust but with a flexible, “breathable” membrane, as shown by investigation on mechanical properties by AFM force measurements in the wet state and under different environmental conditions.  The prospective aim of the project was accomplished through the nonlinear optical induced photochemical reactions which showed the responsible mechanism for NVOC cleavage was the two photon absorption via IR light trigger.

Publications

• 3D-steering and superfocusing of second-harmonic radiation through plasmonic nano antenna arrays J. Laser Appl. 24 (2012) 042005
  Reichenbach, P.; Eng, L.M. ; Georgi, U. ; Voit, B.
  
• Multiphoton photoluminescence contrast in switched Mg:LiNbO3 and Mg:LiTaO3 single crystals, Appl. Phys. Lett. 105 (2014) 122906
  Reichenbach, P.; Kämpfe, T.; Thiessen, A.; Haußmann, A.; Eng, L.M.; Woike, T.
  
• Multiphoton-induced luminescence contrast between antiparallel ferroelectric domains in Mg-doped LiNbO3, J. Appl. Phys. 115 (2014) 213509
  Reichenbach, P.; Kämpfe, T.; Thiessen, A.; Schröder, M.; Haußmann, A.; Woike, T.; Eng, L.M.
  
• Nonlinear optical point light sources through field enhancement at metallic nanocones, Optics Express 22 (2014) 15484
  Reichenbach, P.; Horneber, A.; Gollmer, D.A.; Hille, A.; Mihaljevic, J.; Schäfer, Kern, D.P.; Meixner, A.J.; Zhang, D.; Fleischer, M.; Eng, L.M.
  
• Optical three-dimensional profiling of charged domain walls in ferroelectrics by Cherenkov second-harmonic generation, Phys. Rev. B 89 (2014) 035314
  Kämpfe, T.; Reichenbach, P.; Schröder, M.; Haußmann, A.; Woike, T.; Soergel, E.; Eng, L.M.
  
• Optical antennae for near field induced nonlinear photochemical reactions of photolabile azo- and amine groups, in: Optically Induced Nanostructures: Biomedical and Technical Applications; König, K., Ostendorf, A., Eds.; De Gruyter: Berlin, 2015; pp 267-281
  Reichenbach, P.; Georgi, U.; Oertel, U.; Kämpfe, T.; Nitzsche, B.; Voit, B.; and Eng, L. M.
  
• Immobilized Multifunctional Polymersomes on Solid Surfaces: Infrared Light-Induced Selective Photochemical Reactions, pH Responsive Behavior and Probing Mechanical Properties under Liquid Phase, ACS Applied Materials and Interfaces, 2016, 8 (24), pp 15788–15801
  Iyisan, B.; Janke, A.; Reichenbach, P.; Eng, L. M.; Appelhans, D.; Voit, B.
  
• Multifunctional and Dual-Responsive Polymersomes as Robust Nanocontainers: Design, Formation by Sequential Post-Conjugations, and Ph-Controlled Drug Release. Chem. Mater. 2016, 28, 1513-1525
  Iyisan, B.; Kluge, J.; Formanek, P.; Voit, B.; Appelhans, D.
  
• Time-resolved photo-luminescence spectroscopy of NbNb4+ and O- polarons in LiNbO3 single crystals, Phys. Rev. B 93 (2016)
  Kämpfe, T.; Reichenbach, P.; Haußmann, A.; Thiessen, A.; Woike, T.; Steudtner, R.; Eng, L.M.