Project Details
SFB 628: Functional Membrane Proteomics - From Transport Machineries to Dynamic Assemblies and Networks
Subject Area
Biology
Term
from 2003 to 2007
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5485691
Membrane proteins are the last frontier in the biology of the cell. Determining the structure and understanding the function of membrane proteins remains one of the central challenges in the post-genomic era. At present, the structures of no more than 30 different membrane proteins of 13 families have been determined. This small number is in strikingly contrast to the host of membrane proteins in the genomes sequenced so far. Irrespective of the organism, 25-30% of all genes are predicted to encode membrane proteins. More than half of these are thought to be complex membrane proteins with three or more membrane-spanning segments which carry out essential cellular reactions. To understand the functions of membrane proteins in detail and to apply this knowledge to the development of new therapies and future technologies, it will be necessary to identify and characterise the key protein components, to determine their structure at high resolution and to study their function and molecular interactions in the cell. In the Collaborative Research Centre, we will provide a broad, comprehensive approach in which the participating groups at the Goethe University and at the Max Planck Institutes of Biophysics and of Brain Research will combine forces to find out as much as possible about the identity, expression, structure, function and molecular interactions of membrane proteins and their many fundamental roles in the cell. We will use a wide spectrum of methods to examine diverse biological systems, ranging from the identification and characterisation of gene products, crystallographic and functional studies of individual proteins and protein families, to the investigation of functional networks of membrane proteins in mammalian cells and their relevance to human disease. Since membrane proteins are the least understood elements of the molecular networks that control cellular functions, interaction and evolution, we are confident that the Collaborative Research Centre will make major contributions to our understanding of fundamental cellular processes.
DFG Programme
Collaborative Research Centres
International Connection
USA
Completed projects
- A01 - Structure and Function of Arcaeal Membrane Transporters (Project Head Kühlbrandt, Werner )
- A02 - Function of the Transport Complex TAP (Project Heads Abele, Rupert ; Tampé, Robert )
- A03 - Solid-state NMR on ABC Multidrug Transporter LmrA (Project Head Glaubitz, Clemens )
- B01 - Differential Partners and Dynamics of Membrane Protein Shrew-1 in Polarized and Non-Polarized Cells (Project Head Starzinski-Powitz, Anna )
- B02 - Viral and Instrinsic Modulators (Project Head Tampé, Robert )
- B04 - Function of Reggie Proteins in Cell Adhesion and Membrane Receptor Trafficking (Project Head Tikkanen, Ritva )
- B05 - Dynamics of Type I Interferon Receptor Assembly (Project Head Piehler, Jacob )
- B06 - NMR Investigations of Mammalian Rhodopsin and of Membrane-associated Protein GARP2 (Project Head Schwalbe, Harald )
- C01 - Structural Membrane Proteomics and Genomics Using the Hyperthermophilic Eubacterium Aquifex aeolicus (Project Head Michel, Hartmut )
- C02 - Characterization of Membrane Protein Complexes (Project Heads Karas, Michael ; Schägger, Hermann )
- C03 - Proteomics of Large Membrane Protein Assemblies: The Accessory Subunits of Mitochondrial Complex I (Project Head Brandt, Ulrich )
- C04 - Structural and Functional Investigation of Membrane Proteins by Combined Approaches of Cell-Free Expression and NMR Spectroscopy (Project Heads Bernhard, Frank ; Dötsch, Volker )
- C05 - Proteomic Analysis of Postsynaptic Receptor Complexes (Project Head Betz, Heinrich )
- C06 - The Synaptic Vesicle Proteome: Identification and Functional Characterization of Novel Constituents (Project Heads Volknandt, Walter ; Zimmermann, Herbert )
- C07 - Functional Analysis of Novel Proteins Associated with Nicotinic Acetylcholine Receptors and Synaptic Vesicles in Caenorhabditis elegans (Project Head Gottschalk, Alexander )
- P04 - Protein Transport Across Biological Membranes - the HlyB/HlyA System from E.coli (Project Head Schmitt, Lutz )
- P05 - Structural and Functional Analysis of Protein Translocation (Project Head Collinson, Ian )
- P06 - Molecular Cross Talk at the Endothelial Membrane (Project Head Müller-Esterl, Werner )
- P07 - Funkctional Dynamics of Shrew-1 in Epithelial Adherens Junctions (Project Head Starzinski-Powitz, Anna )
- P08 - Viral Inhibitors of the Antigen Processing Machinery (Project Head Tampé, Robert )
- P10 - Dynamics of Type I-Interferon Receptor Assembly (Project Head Piehler, Jacob )
- P11 - High Resolution Spectroscopy Investigations of T-Cell Activation and G Protein Coupled Receptors (Project Head Schwalbe, Harald )
- P12 - Membrane Proteomics using the Hyperthermophilic Eubacterium Aquifex Aeolicus (Project Head Michel, Hartmut )
- P13 - Characterization of Membrane Protein Complexes (Project Heads Karas, Michael ; Schägger, Hermann )
- P14 - Proteomics of Large Membrane Protein Assemblies: the Accessory Subunits of Mitochondrial Complex I (Project Head Brandt, Ulrich )
- P15 - Proteomic Analysis of Postsynaptic Receptor Complexes (Project Heads Betz, Heinrich ; Kneussel, Ph.D., Matthias )
- P16 - The Synaptic Vesicle Proteome under conditions of Rest and Activation (Project Heads Volknandt, Walter ; Zimmermann, Herbert )
- P17 - Proteomic and funkctional analysis of pre- and post-synaptic componentes in Caenorhabditis elegans (Project Head Gottschalk, Alexander )
- P18 - Strukturelle und funktionelle Analyse integraler Membranproteine (Project Head Dötsch, Volker )
- P19 - Functional characterization of the reggie protein family: posttranslational modifications and signaling partners (Project Head Tikkanen, Ritva )
- Z01 - Zentrale Aufgaben (Project Head Tampé, Robert )
Applicant Institution
Goethe-Universität Frankfurt am Main
Participating Institution
Max-Planck-Institut für Biophysik; Max-Planck-Institut für Hirnforschung
Spokesperson
Professor Dr. Robert Tampé