SFB 593:  Mechanisms of Cellular Compartmentation and the Relevance for Disease

• Subject Area: Medicine ; Biology
• Term: from 2003 to 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5485539

The central goal of the Collaborative Research Centre is the molecular understanding of cellular compartmentation and its relevance for the development of disease. The Research Centres will investigate important questions of modern cell biology such as the intracellular distribution of proteins, RNA, and metals between various organelles, the assembly of plasma membrane transporters, the intracellular maturation and assembly of highly pathogenic viruses, and the processes of compartmentation of intracellular pathogenes. The Collaborative Research Centre is further interested in how the molecular function of protein complexes ("molecular machines") is utilised for the generation, structural organisation and maintenance of intracellular compartments. In addition to these basic problems, research will be focused at the elucidation of the consequences of pathogenic disturbance of cellular compartmentation for the properties of the individual compartments, for cellular homeostasis and for the entire organism. The Collaborative Research Centre will use the classical techniques of biochemistry, cell biology, genetics, molecular biology, parasitology, physiology, structural biology and virology. On top of these methods the modern techniques of "genomics" and proteomics" will be applied. The major research objects are mammalian cell culture and model organisms such as yeast and transgenic mice. Appropriate high security laboratories will be used for the work with highly pathogenic viruses and for examination of genetically engineered parasites and viruses. The comparison of cellular processes in healthy and pathogenically altered cells will enable the Research Centres to unravel numerous poorly defined pathways at a molecular level and describe the alterations in the diseased state.

• DFG Programme: Collaborative Research Centres
• International Connection: France

Completed projects

• A01 - The role of mitochondria in the maturation of cytosolic and nuclear iron-sulfur proteins (Project Head Lill, Roland )
• A02 - Mechanism and Regulation of the Cellular Compartmentalization of Iron between Mitochondria and Cytosol (Project Head Mühlenhoff, Ulrich )
• A03 - Composition, cellular targeting and regulation of protein kinase-linked cation channel complexes of the TRPM family (Project Heads Gudermann, Thomas ;  Schlingmann, Karl Peter ;  Waldegger, Siegfried )
• A04 - Intracellular traffic of potassium channels (Project Head Daut, Jürgen )
• A06 - The role of specific mRNA binding proteins during export from the nucleus to the cytoplasm (Project Head Krebber, Heike )
• A07 - Compartmentaion of Myc Degradation (Project Head Eilers, Martin )
• A09 - Identification of vesicle components involved in apical protein transport (Project Head Jacob, Ralf )
• A10 - Importance and regulation of edocytoic recycling and bidirectional endosome motility (Project Head Steinberg, Gero )
• A11 - Endothelial caveolae as functional microcompartments of mechanotransduction and their role in hypertension and renal failure (Project Head Hoyer, Joachim )
• A12 - Compartment-specific phosphoinositide dynamics in a central neuron (Project Head Oliver, Dominik )
• A13 - Spatiotemporal and structural aspects of G protein mediated signal transduction (Project Head Bünemann, Moritz )
• A14 - Nuclear localization and regulation by sumoylation of SCAI (Project Head Grosse, Robert )
• A15 - Molecular mechanismus underlying regulated transport and processing of neuronal microRNAs in the postsynaptic compartment (Project Head Schratt, Ph.D., Gerhard Martin )
• A16 - The importance of TRPMI and TRPM3 channel proteins for the establishment and function of the postsynaptic dendritic compartment of retinal bipolar cells (Project Head Oberwinkler, Johannes )
• B01 - Interactions of influenza virus polymerase and hemagglutinin with host factors and their roles in pathogenesis and host adaptation (Project Head Klenk, Hans-Dieter )
• B02 - Proteases of the Respiratory Tract Activating Influenza Viruses: Identification, Characterization and Cellular Compartmentalization (Project Heads Böttcher-Friebertshäuser, Eva ;  Garten, Wolfgang )
• B03 - Morphogenese und Transport von Nukleokapsidkomplexen des Marburg-Virus (Project Head Becker, Stephan )
• B04 - Reverse genetics system for Ebola virus - molecular basis of high pathogenicity (Project Head Volchkov, Viktor )
• B05 - Struktur und Funktion von viralen Matrixproteinen (Project Head Weissenhorn, Winfried )
• B07 - Biogenesis of the parasitophorous vacuole of apicomplexan parasites in human erythrocytes (Project Head Lingelbach, Klaus )
• B09 - Proteintransport in komplexe Plastiden (Project Head Maier, Uwe Gallus )
• B10 - The role of selected Ustilago maydis proteins during pathogenesis (Project Head Kahmann, Regine )
• B11 - Role of the endosomal compartment in Nipah virus replication (Project Head Maisner, Andrea Susanne )
• B12 - Morphogenesis and transport of filovims nucleocapsids (Project Head Becker, Stephan )
• B13 - Disturbance of the nuclear and nucleolar compartments by a viral interferon antagonist (Project Head Weber, Friedemann )
• N01 - Roles of differentially localized glutaredoxin 2 isoforms (Project Head Lillig, Christopher Horst )
• N01 - N01 - Roles of differentially localized glutaredoxin 2 isoforms (Project Head Lillig, Christopher Horst )
• Z01 - Zentrale Aufgaben (Leistungen für alle Teilprojekte) (Project Head Lill, Roland )
• Z02 - Protein analysis (Project Head Lingelbach, Klaus )

• Applicant Institution: Philipps-Universität Marburg
• Participating Institution: European Molecular Biology Laboratory (EMBL) Grenoble; Max-Planck-Institut für terrestrische Mikrobiologie; Université Claude Bernard Lyon 1
• Spokesperson: Professor Dr. Roland Lill