Role of plant SRO proteoforms in tolerance to oxidative and salinity stress (B05)

• Subject Area: Plant Physiology ; Biochemistry ; Plant Biochemistry and Biophysics ; Biophysics ; Plant Genetics and Genomics ; Structural Biology ; Plant Cell and Developmental Biology ; Cell Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 514901783

B05 investigates two orthologous proteins from Arabidopsis and wheat, studying functional differences within a species and between orthologous proteins. RADICAL-INDUCED CELL DEATH 1 (AtRCD1) andits wheat ortholog influence plant tolerance to oxidative stress and/or salinity. Analyses focus on nsSNPs in the essential WWE-PARP domain, crucial for oligomer formation and binding to stress-induced poly(ADP-ribose) chains. Methodologies compare proteoforms biochemically and structurally (x-ray crystallography), revealing differences in WWE and PARP domain cooperation and their impact on oxidative stress tolerance.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1664: Plant Proteoform Diversity - SNP2Prot
• Applicant Institution: Martin-Luther-Universität Halle-Wittenberg
• Project Heads: Professor Dr. Milton T. Stubbs; Dr. Lennart Wirthmüller