Natural allelic variation of PI4P 5-kinases modulating plasma membrane-associated processes in plants (B01)

• Subject Area: Plant Physiology ; Biochemistry ; Plant Biochemistry and Biophysics ; Biophysics ; Structural Biology ; Plant Cell and Developmental Biology ; Cell Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 514901783

B01 explores natural allelic variation in PIP5-kinases, pivotal for stress responses through PIP2 production. Trigger-induced kinase phosphorylation and phosphorylation pattern variations offer potential adaptation to environmental changes. The project employs a comprehensive approach, integrating biochemical, structural, cell biological, and physiological analyses. Purified proteoforms undergo XL-MS scrutiny for folding patterns, guiding homology models. Assessing the impact of phosphorylation pattern variations in planta via physiological assays provides insights into the plasma membrane's role in stress response adaptation.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1664: Plant Proteoform Diversity - SNP2Prot
• Applicant Institution: Martin-Luther-Universität Halle-Wittenberg
• Project Heads: Professor Dr. Ingo Heilmann; Professorin Dr. Andrea Sinz