Investigating the Therapeutic Potential of Infrared Light Technology: Improving Mitochondrial Quality Control in Aging Human Cells.

• Applicant: Tasnim Arroum, Ph.D.
• Subject Area: Cell Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 545044286

In this research project, my aim is to investigate mitochondrial dysfunction related to aging of human cells. For this endeavor, we are employing an innovative approach that utilizes inhibitory near-infrared light (IRL) wavelengths, specifically 750 nm and 950 nm, to photomodulate mitochondrial function and improve mitochondrial quality control in aging human cells. This modulation occurs through the inhibition of cytochrome c oxidase, thereby partially inhibiting mitochondrial respiration, and, in parallel, the production of reactive oxygen species, known key drivers for inflammation, DNA damage, and cellular senescence. Our project employs a multifaceted approach through three objectives. Firstly, we will establish an in vitro cell culture model, which will utilize primary human fibroblasts from both young and old donors sourced from the NIH National Institute of Aging (NIA), housed at the Coriell Institute for Medical Research, USA. We will implement successful read-out techniques and advanced molecular assays to measure senescence-related markers and assess cellular bioenergetics. The second objective entails the induction of senescence in 1) human fibroblast cell lines, using diploid normal cells for replicative senescence, and 2) immortalized human fibroblasts for chemically induced senescence. Finally, the third objective focuses on evaluating the impact of inhibitory IRL wavelengths (750 nm and 950 nm) on delays in senescence phenotype development and potential rejuvenation effects by improving mitochondrial quality control and by reducing inflammation. This project aspires not only to contribute to our understanding of cellular senescence but also to introduce an innovative strategy using near-infrared light treatment for promoting healthy aging.

• DFG Programme: WBP Fellowship
• International Connection: USA
• Host: Professor Maik Hüttemann, Ph.D.