Transcript quality effects of RNAPII speed variation (A01)

• Subject Area: Cell Biology ; Bioinformatics and Theoretical Biology ; Nephrology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 520471345

Recently, we have shown that an age-associated increase in average RNAPII elongation speed impairs the production of mRNA. However, we lack understanding about the molecular factors contributing to specific splicing changes and other mRNA sequence errors. We will create cellular models of accelerated and slowed-down RNAPII and profile those models by separately analyzing their nuclear and cytoplasmic transcriptomes. We will investigate the consequences of speed changes for transcript structures and protein levels in cellular models, mouse models and human kidney samples.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1678: Systems-level consequences of fidelity changes in mRNA and protein biosynthesis
• Applicant Institution: Universität zu Köln
• Project Heads: Professor Dr. Andreas Beyer; Professor Dr. Roman-Ulrich Müller