Study on the biosynthetic mechanism of signalling molecules controlling secondary metabolism in Streptomyces species

• Applicant: Professorin Dr. Eriko Takano
• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2004 to 2008
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5427505

Streptomyces produce a wide variety of secondary metabolites including most of the antibiotics that are commercially available. Small signalling molecules called g-butyrolactones are found exclusively in more than 60% of Streptomyces species and play an important role in the regulation of antibiotic production. In spite of the fact that the first g-butyrolactone was identified 23 years ago, the biosynthesis pathway for these molecules has not been clearly identified. We would like to combine the expertise of the two groups to determine the g-butyrolactone biosynthesis genes. The Japanese group is specialised in biochemistry and genetics of S. virginiae and has a vast experience and knowledge of the virginiae butanolides. In Germany, not only is there experience and knowledge in molecular aspects including transcriptomics of the model Streptomyces S. coelicolor, but also knowledge of the Streptomyces coelicolor butyrolactones. By approaching this important question from two different Streptomyces species, we will be able to learn the communal biosynthesis pathway and also the differences which will aid us in further understanding the role of the different g-butyrolactones in each Streptomyces species and how they have diverged from each other.

• DFG Programme: Research Grants
• International Connection: Japan, Netherlands
• Participating Person: Professor Dr. Hiroshi Kinoshita