Biochemical and spectroscopic characterization of blue light receptors with LOV and BLUF-domain-type chromophores from microalgae and purple bacteria

• Applicants: Professor Dr. Peter Hegemann; Professorin Dr. Ilme Schlichting
• Subject Area: Biophysics
• Term: from 2004 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5470629

Final Report Abstract

No abstract available

Publications

• (2008) Algal sensory photoreceptors, Ann. Rev. Plant Biol. 59, 167-189
  Hegemann, P.
  (See online at https://doi.org/10.1146/annurev.arplant.59.032607.092847)
• (2008) Hydrogen bond switching among flavin and amino acid side chains in the BLUF photoreceptor probed by ultrafast infrared spectroscopy. Biophys. J. 95, 4790-4802
  Bonetti, C., Mathes, T.. van Stokkum, I. H. M., Mullen, K. M., Groot, M.-L., van Grondelle, R., Hegemann, P., and Kennis, J.T.M.
  (See online at https://doi.org/10.1529/biophysj.108.139246)
• (2008) Molecular models predict light-induced glutamine tautomerization in BLUF photoreceptors. Biophys. J. 94, 3872-3879
  Domratcheva, T., Grigorenko, B.L., Schlichting, I., Nemukhin, A.V.
  (See online at https://doi.org/10.1529/biophysj.107.124172)
• (2008) Sensory photoreceptors and Light Control of Flagellar Activity. Chlamydomonas Source Book. Vol. III Ed.G. Witman, pp. 391-425
  Hegemann, P. and Berthold, P.
  (See online at https://doi.org/10.1016/B978-0-12-370873-1.00050-2)
• (2009) A genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461, 104-108
  Wu, Y.I., Frey, D., Lungu, O.I., Jaehrig, A., Schlichting, I., Kuhlmann, B., Hahn, K.M.
  (See online at https://doi.org/10.1038/nature08241)
• (2009) Absorption and Emission Spectroscopic Characterisation of 8-Amino-Riboflavin. Chemical Physics 264, 19 - 30
  Tyagi, A., Zirak, P., Penzkofer, A., Mathes, T., Hegemann, P., Mack, M., Ghisla, S.
  (See online at https://doi.org/10.1016/j.chemphys.2009.08.005)
• (2009) Absorption and emission spectroscopic characterization of BLUF Protein Slr1694 from Synechocystis sp. PCC6803 with Roseoflavin Cofactor. J. Photochem. Photobiol. B. Biology 97, 61-70
  Zirak, P., Penzkofer, A., Mathes, T., Hegemann, P.
  (See online at https://doi.org/10.1016/j.jphotobiol.2009.08.002)
• (2009) In vivo generation of flavoproteins with modified cofactors. J. Mol. Biol. 385, 1511-18
  Mathes, T., Vogl, C., Stolz, J., and Hegemann, P.
  (See online at https://doi.org/10.1016/j.jmb.2008.11.001)
• (2009) Mechanistic insights in Light-induced cAMP production by photoactivated adenylyl cyclase alpha (PACa) Biological Chemistry 390, 1105-1111
  Looser, J., Schröder-Lang, S., Hegemann, P., and Nagel, G.
  (See online at https://doi.org/10.1515/BC.2009.132)
• (2009) Photo-dynamics of Roseoflavin and Riboflavin in Aqueous and Organic Solvents. Chemical Phys. 358, 111-122
  Zirak, P., Penzkofer, A., Mathes, T., and Hegemann, P.
  (See online at https://doi.org/10.1016/j.chemphys.2008.12.026)
• (2009) Photodynamics of blue-light-regulated phosphodiesterase BlrP1 protein from Klebsiella pneumoniae and its photoreceptor BLUF Chemical Physics 354, 130 - 141
  Tyagi, A., Penzkofer, A., Griese, J., Schlichting, I., Kirienko, N.V. Gomelsky, M.
  (See online at https://doi.org/10.1016/j.chemphys.2008.10.003)
• (2009) Structure and Mechanism of a Bacterial Light-Regulated Cyclic Nucleotide Phosphodiesterase. Nature, 459: 1015-1018
  Barends, T.R.M., Hartmann, E., Griese, J.J., Beitlich, T., Kirienko, N.V., Ryjenkov, D.A., Reinstein, J., Shoeman, R.L., Gomelsky, M., and Schlichting, I.
  (See online at https://doi.org/10.1038/nature07966)
• (2009) The role of key amino acids in the photoactivation pathway of the Synechocystis Slr1694 BLUF domain. Biochemistry. 48(48):11458-69
  Bonetti, C., Stierl, M., Mathes, T., Stokkum, I., Mullen, K., Cohen-Stuart, T., Van Grondelle, R., Hegemann, P., Kennis, J.
  (See online at https://doi.org/10.1021/bi901196x)
• 2009) The role of key amino acids in the photoactivation pathway of the Synechocystis Slr1694 BLUF domain. Biochemistry. 48(48):11458-69
  Bonetti, C., Stierl, M., Mathes, T., Stokkum, I., Mullen, K., Cohen-Stuart, T., Van Grondelle, R., Hegemann, P., Kennis, J.
  (See online at https://doi.org/10.1021/bi901196x)
• (2010) Blue Light Induces Global and Localized Conformational Changes in the Kinase Domain of Full-Length Phototropin. Biochemistry. 49, 1024 – 1032
  Pfeifer A, Mathes T, Lu Y, Hegemann P, Kottke T.
  (See online at https://doi.org/10.1021/bi9016044)
• (2010) Blue Light Induces Global and Localized Conformational Changes in the Kinase Domain of Full-Length Phototropin. Biochemistry. 49, 1024 – 1032
  Pfeifer A, Mathes T, Lu Y, Hegemann P, Kottke T.
  (See online at https://doi.org/10.1021/bi9016044)
• (2010) Hindered Rotation of a Cofactor Methyl Group as a Probe for Protein-Cofactor Interaction JACS 132, 8935-8944
  Brosi, R., Illianorov, B., Mathes, T., Fischer, M., Bacher, A., Hegemann, P., Bittl, R., Weber, S. and Schleicher, E.
  (See online at https://doi.org/10.1021/ja910681z)
• (2010) Photodegradation behaviour of roseoflavin in some equous solutions. Chemical Physics Chemical Physics 369 , 27 - 36
  Tyagi, A., Penzkofer, A., Mathes, T., Hegemann, P.
  (See online at https://doi.org/10.1016/j.chemphys.2010.02.004)