Project Details
Projekt
Multiparametric functional analysis of monocytic and lymphocytic subpopulations after severe multiple injury
Applicant
Professor Dr. Eugen Faist
Subject Area
Orthopaedics, Traumatology, Reconstructive Surgery
Term
from 2003 to 2005
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5409001
Severe tissue trauma results in a general inflammatory immune response, the "systemic inflammatory response syndrome" (SIRS), representing an overall inflammatory reaction of the immune system. There are less clinical data concerning the early phase after severe trauma, which is characterized by the battle of the individuum with the initial trauma, compared to the late posttraumatic complications with reproducible cytokine levels. The sole determination of systemic cytokine levels does not allow to draw conclusions on the synthesis and secretion of cytokines in vivo due to their short half-life periods. Moreover, like the analysis of mRNA it remains questionable whether the heterogenity of cytokine expressing cells is responsible for the coexpression of different cytokines or if different cytokines are coexpressed in the individuell cell. So far the flowcytometric analysis of secreted proteins was feasible only in a restricted manner. Today they can be detected either in fixed cells intracellularly, i.e. prior to the secretion, or extracellularly in viable cells. These procedures allow the quantitative and kinetic analysis of the expression of the cytokine genes correlated with other parameters and on a per cell basis. Thus it is the aim of the study to determine the individual status of the immune system (hypersensitivity/hyporeactivity) after severe tissue trauma via the flowcytometric analysis of intra- and extracellular cytokines (immunomonitoring). Thereby a further objective is to shed light on the way the systemic cytokine levels are made up in the organism after severe trauma and to clarify to which extent T-lymphocytes and monocytes or their subpopulations, respectively contribute to the secretion of these mediators.
DFG Programme
Priority Programmes
