Molekulare Analyse von Eisen- und Zink-Aufnahme Systemen in Escherichia coli mit Schwerpunkt ZupT

• Applicant: Privatdozent Dr. Gregor Grass
• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2003 to 2009
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5403028

The ZupT protein is the first described member of the ZIP (Zrt- and Irt-related protein) family of iron and zinc transportes in prokaryotes. ZIP-like proteins occur in all domains of life, including man, and are important factors for iron, manganese and zinc homeostasis. The goals of our proposal are (i) to understand the physiological role of ZupT amongst other ferrous transporters in Escherichia coli, (ii) to identify amino acid residues in ZupT important for function and metal specifity, (iii) to characterize ZupT mediated transport biochemically in cooperation with Christopher Rensing (University of Arizona, Tucson, USA) and (iv) to identify new iron transporters employing DNA-micro array analysis for E. coli.

• DFG Programme: Research Grants
• Participating Person: Professor Dr. Dietrich H. Nies