Signaling Proteomics: Die Analyse von Signaltransduktionsnetzwerken tierischer und pflanzlicher Rezeptorkinasen mit Hilfe der Proteinmassenspektrometrie
Final Report Abstract
Macronutrients and metabolites, such as carbon in the form of sugars, nitrogen in the form of nitrate, ammonium and nitrogen-containing metabolites, and phosphate are not only essential for plant growth but also have signaling functions in metabolism and development. While the transcriptional responses to changes in these metabolites and nutrients have been fairly well studied by various research groups, the signal transduction events upstream of transcription are still barely revealed. Therefore, we focus on the analysis of proteins, which through changes in activity and abundance lead to short term adaptive responses. In addition, the change in protein modification status and abundance may also serve in signaling pathways. Therefore, the research focus of the group in the past years has been on the system-wide analysis of dynamic changes in membrane protein abundance and protein modification under changing nutrient regimes. Our aim is to understand global mechanisms of how plants adjust to changing environments (i.e. nutrients) within short time frames. In addition, we have contributed to studies of carbon and nutrient responses of other groups by using proteomics and phosphoproteomics to search for changes in the protein and phosphoprotein profile.
Publications
- (2008) Mass spectrometry based proteomics as a tool for the analysis of proteinprotein interactions in signaling processes. In: Bögre L, Beemster TGS (eds.) Plant Cell Monographs 10: Plant Growth Signalling. Springer Verlag, Berlin, 315-329
Schulze W
- (2009) Feedback inhibition of ammonium uptake by a phosphodependent allosteric mechanism. The Plant Cell 21 (11): 3610-3622
Lanquar V, Loque D, Hörmann F, Yuan L, Bohner A, Engelsberger WR, Lalonde S, Schulze WX, von Wiren N, Frommer WB
- (2009) Plant phosphoproteomics: An update. Proteomics 9: 964-988
Kersten, B, Agrawal, GK, Durek P, Neigenfind J, Schulze WX, Walther D, Rakwal R
- (2009) Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis. Molecular Systems Biology 5 (314): E1-E17
Piques MC, Schulze WX, Höhne M, Usadel B, Gibon Y, Rohwer J, Stitt M
- (2010) Identification of protein stability determinants in chloroplasts. Plant Journal 63: 636-650
Apel W, Schulze WX, Bock R
- (2010) PAMP-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity. Journal of Biological Chemistry, 285 (50): 39140-39149
Keinath NF, Kierszniowska S, Lorek J, Bourdais G, Kessler SA, Asano H, Grossniklaus U, Schulze WX, Robatzek S, Panstruga R
- (2010) PhosPhAt: The Arabidopsis thaliana phosphorylation site database. An update. Nucleic Acids Research 38: D828-834
Durek P, Schmidt R, Heazlewood JL, Jones A, MacLean D, Nagel A, Kersten B, Schulze WX
- (2010) Proteome-wide survey of phosphorylation patterns affected by nuclear DNA polymorphisms in Arabidopsis thaliana. BMC Genomics 11: 411
Riano-Pachon DM, Kleesen S, Neigenfind J, Durek P, Weber E, Engelsberger WR, Walther D, Selbig J, Schulze WX, Kersten B
- (2010) Proteomic approaches to understand protein phosphorylation in pathway modulation. Current Opinion in Plant Biology 13: 1-8
Schulze WX
- (2010) Quantitation in Mass-Spectrometry-Based Proteomics. Annual Review of Plant Biology 61: 491-516
Schulze WX, Usadel B
- (2011) Identification of lily pollen 14-3-3 isoforms and their sub-cellular and time dependent expression profile. Biological Chemistry, 392: 249–262
Pertl H, Rittmann S, Schulze WX, Obermeyer G
- (2011) MASCP Gator: An aggregation portal for the visualization of Arabidopsis proteomics data. Plant Physiology, 155 (1):259-270
Joshi HJ, Hirsch-Hoffmann M, Bärenfaller K, Gruissem W, Baginsky S, Schmidt R, Schulze WX, Sun Q, van Wijk KJ, Egelhofer V, Wienkoop S, Weckwerth W, Bruley C, Rolland N, Toyoda T, Nakagami H, Jones AME, Briggs SP, Castleden I, Tanz SK, Millar H, Heazlewood JL
