Reversible Long-range PCET in Ribonucleotide Reductases (B02)

• Subject Area: Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry ; Biochemistry ; Structural Biology
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 510228793

B02 aims to elucidate the mechanism of coupled proton and electron transfer across the / subunit interface of the enzyme E. coli ribonucleotide reductase (RNR). Advanced hyperfine spectroscopy (ENDOR, ELDOR), along with biochemical labelling and biophysical methods, such as high-resolution protein crystallography and cryo-EM, will be applied to derive the structural basis for water-assisted PCET over long distances (> 30 Å), as a blueprint for efficient PCET at low driving force. B02 collaborates with projects A01/A03/B03/B04 (EPR spectroscopy) and with B04 (theory).

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1633: Pushing Electrons with Protons – Unifying Multi-Electron Redox Catalysis by Proton-Coupled Electron Transfer
• Applicant Institution: Georg-August-Universität Göttingen
• Project Heads: Professorin Dr. Marina Bennati; Professor Dr. Kai Tittmann