Chemische und zellbiologische Steuerung der in vitro-Remodellierung von Hydroxylapatit
Final Report Abstract
Within the priority program a co-culture model, consisting of an artificial extracellular bone matrix (mineralized collagen), osteoblasts and osteoclasts could be developed which allows insights in biomineralization phenomena and remodeling mechanisms of mineralized tissue. The effects of osteocalcin and phosphoserine (the latter as a model for the non-colleagenous protein osteopontin) on collagen fibril reassembly, mineralization – and also cellular behavior were studied using this system. The formation of osteoclast-like cells from human monocytes depends very much on the kind of substrate they are attached to. The ratio of multinucleated to mononuclear cells differs between glass, plastic and hydroxyapatite containing materials. Addition of phosphoserine, the mineral-binding amino acid in the bone matrix protein osteopontin, leads to a changed microstructure of hydroxyapatite materials, absence of multinucleated cells and to a decrease in transcription levels of osteoclastic genes such as TRAP and cathepsin K. In contrast, osteocalcin, that also changes microstructure, does not affect transcription levels in the tested concentrations.
Publications
- Influence of osteocalcin and collagen I on the mechanical and biological properties of Biocement D. Biomol Eng. 2002, 19, 227-231
B. Knepper-Nicolai B, A. Reinstorf A, I. Hofinger, K. Flade, R. Wenz, W. Pompe
(See online at https://doi.org/10.1016/S1389-0344(02)00036-9) - Phosphoserine – a convenient compound for modification of calcium phosphate bone cement collagen composites. J. Mater. Sci. Mater. Med. 2004, 15, 451-455
A. Reinstorf, M. Ruhnow, M. Gelinsky, W. Pompe, U. Hempel, K.-W. Wenzel, P. Simon
(See online at https://doi.org/10.1023/B:JMSM.0000021119.14870.3d) - In vitro ossification and remodeling of mineralized collagen I scaffolds. Tissue Eng. 2006, 12, 949-958
H. Domaschke, M. Gelinsky, B. Burmeister, R. Fleig, Th. Hanke, A. Reinstorf, W. Pompe, A. Rösen-Wolff
(See online at https://doi.org/10.1089/ten.2006.12.949) - O-phospho-L-serine modified calcium phosphate cements – material properties, in vitro and in vivo investigations. Materialwiss. Werkstofftech. 2006, 37, 491-503
A. Reinstorf, U. Hempel, F. Olgemöller, H. Domaschke, W. Schneiders, R. Mai, B. Stadlinger, A. Rösen-Wolff, S. Rammelt, M. Gelinsky, W. Pompe
(See online at https://doi.org/10.1002/mawe.200600026) - Porous three dimensional scaffolds made of mineralised collagen: preparation and properties of a biomimetic nanocomposite material for tissue engineering of bone. Chem. Eng. J. 2008, 137, 84-96
M. Gelinsky, P. B. Welzel, P. Simon, A. Bernhardt, U. König
(See online at https://doi.org/10.1016/j.cej.2007.09.029) - Crosstalk of osteoblast and osteoclast precursors on mineralised collagen – towards an in vitro model for bone-remodelling. J. Biomed. Mater. Res. A 2010, 95A, 848-856
A. Bernhardt, S. Thieme, H. Domaschke, A. Springer, A. Rösen-Wolff, M. Gelinsky
(See online at https://doi.org/10.1002/jbm.a.32856)
