Project Details
Projekt
Dynamic organisation of the localised ASH1 messenger ribonucleoprotein complex in yeast.
Applicant
Professor Dr. Ralf-Peter Jansen
Subject Area
Cell Biology
Term
from 2001 to 2008
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5468076
Intracellular localisation of messenger RNAs is a widespread mechanism to achieve asymmetric protein distribution. A number of localised mRNAs as well as the nature of their corresponding localisation signals are known. However, little is understood about the proteins that assemble with the mRNA during different stages of localisation or about the dynamic reorganisation of localised mRNA protein-complexes (mRNPs) during nuclear export, cytoplasmic transport and anchoring at the target site. In budding yeast, the mRNA encoding the transcription factor Ash1p is localised during anaphase to one of the two cells emerging from mitosis. Cytoplasmic transport of the mRNA requires a molecular motor, Myo4p, as well as adapter proteins that connect the RNA and the motor. We would like to understand when and how these proteins assemble with ASH1 mRNA and what other predicted but still unknown factors control the ASH1 RNP's nuclear export, cytoplamic localisation and anchoring at the destination site.
DFG Programme
Research Units
