Studying crosstalk dynamics of signaling complexes in extrinsic and intrinsic cell death pathways by MS-based proteomics (A07)

• Subject Area: Cell Biology
• Term: since 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 471011418

Many tumor cells show resistance to the extrinsic and the intrinsic apoptosis pathways, yet their combined activation can override resistance and result in profound cell death induction. Using proteomics and structural mass spectrometry (MS), in particular crosslinking coupled to MS (XL-MS), this project will investigate how dynamic changes in the TRAIL receptor signaling complex and the BCL-2 interactome can sensitize hepatocellular carcinomas to the synergistic induction of apoptosis by TRAIL and chemotherapeutic drugs.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 353: Regulation of cell death decisions
• Applicant Institution: Universität Konstanz
• Project Heads: Professor Dr. Thomas Brunner; Professor Dr. Florian Stengel